Sromová D, Lýsek H
Department of Medical Biology, Medical Faculty, Palacký University, Olomouc, Czechoslovakia.
Folia Parasitol (Praha). 1990;37(1):77-80.
The formation of chitin-protein layer in Ascaris lumbricoides egg-shells was studied using the method of chitin structures visualization by means of derivatives of stilbene-disulfonic acid (Blankophor, Bayer, FRG). Intensive formation of chitin structures in A. lumbricoides egg-shell occurred only in fertilized females in a very short portion of uterus from the site of connection of the oviduct with the uterus up to 25-30 mm distally, i.e. in the section forming about one fifth or less than one sixth of length of adult female uterus. Already 3 mm from the beginning of uterus there was a thin layer on the surface of the fertilized eggs which contained chitin detectable by Blankophor visualization, whereas the chitin-protein layer on the shells could not be demonstrated histologically. The thickness of this chitin-protein complex increased distally. At 25 mm from the beginning of uterus the chitin layer was already complete and its thickness did not increase any more.
利用二苯乙烯二磺酸衍生物（Blankophor，德国拜耳公司）对几丁质结构进行可视化的方法，研究了蛔虫卵壳中几丁质-蛋白质层的形成。蛔虫卵壳中几丁质结构的强烈形成仅发生在受精雌虫子宫中很短的一段，从输卵管与子宫连接处开始，向远端25 - 30毫米处，即形成约占成年雌虫子宫长度五分之一或不到六分之一的部分。从子宫开始处起3毫米处，受精卵表面就有一层薄的、可通过Blankophor可视化检测到几丁质的层，而壳上的几丁质-蛋白质层在组织学上无法显示。这种几丁质-蛋白质复合物的厚度向远端增加。从子宫开始处起25毫米处，几丁质层已经完整，其厚度不再增加。
