Wei Haixiang, Shen Gan, Deng Xiaolong, Lou Dong, Sun Binbin, Wu Hao, Long Long, Ding Tao, Zhao Jian
Orthopedic Department, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
Cell Tissue Bank. 2013 Dec;14(4):699-706. doi: 10.1007/s10561-012-9354-9. Epub 2013 Jan 17.
Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammatory cytokines, such as interleukin 6 (IL-6) play a pivotal role. To investigate the direct role of IL-6 in the chondrogenic differentiation of murine MSCs (mMSCs), we isolate MSCs from the murine bone marrow, and induce MSCs chondrogenesis with different concentrations of IL-6 in vitro. Through detecting the histological and histochemical qualities of the aggregates, we demonstrate that IL-6 inhibited the differentiation of MSCs into chondrocytes in the dose-dependence manner. These findings suggest that possible strategies for improving the clinical outcome of cartilage repair procedures.
