Tian Ling, Wang Rongsheng E, Chang Yie-Hwa
Mediomics, LLC, St Louis, MO, USA.
Antivir Ther. 2012;17(7 Pt B):1437-42. doi: 10.3851/IMP2469. Epub 2012 Dec 7.
Molecular PINCERs(®) are antibody-based protein sensors that allow rapid and sensitive detection of specific proteins in solution. The sensors allow quick detection of a target, with sensitivity and specificity approaching that of conventional immunoassays, but require no sample manipulation other than mixing of the test sample with sensors. Combining the PINCER assay with immunoprecipitation allows quick and sensitive detection of anti-HCV immunoglobulin (Ig)G and IgM in human serum. This improvement is achieved through binding of anti-HCV IgG and IgM from serum to HCV-antigen-immobilized microspheres, followed by quantification of the IgG and IgM using molecular PINCERs. The PINCER-based assay is cost-effective, sensitive and has potential for clinical diagnostic use.
分子钳（PINCERs®）是基于抗体的蛋白质传感器，可对溶液中的特定蛋白质进行快速灵敏的检测。这些传感器能够快速检测目标物，其灵敏度和特异性接近传统免疫测定法，但除了将测试样品与传感器混合外，无需对样品进行其他处理。将分子钳检测法与免疫沉淀相结合，能够快速灵敏地检测人血清中的抗丙型肝炎病毒（HCV）免疫球蛋白（Ig）G和IgM。这一改进是通过将血清中的抗HCV IgG和IgM与固定有HCV抗原的微球结合，然后使用分子钳对IgG和IgM进行定量来实现的。基于分子钳的检测方法具有成本效益、灵敏度高，具有临床诊断应用潜力。
