College of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia Autonomous Region, China.
World J Gastroenterol. 2012 Dec 21;18(47):7087-92. doi: 10.3748/wjg.v18.i47.7087.
To improve the outcome of orthotopic transplantation in a mouse model, we used an absorbable gelatin sponge (AGS) in nude mice to establish an orthotopic implantation tumor model.
MHCC-97L hepatocellular carcinoma (HCC) cells stably expressing the luciferase gene were injected into the subcutaneous region of nude mice. One week later, the ectopic tumors were harvested and transplanted into the left liver lobe of nude mice. The AGS was used to establish the nude mouse orthotopic implantation tumor model. The tumor suppressor gene, paired box gene 5 (PAX5), which is a tumor suppressor in HCC, was transfected into HCC cells to validate the model. Tumor growth was measured by bioluminescence imaging technology. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and histopathology were used to confirm the tumorigenicity of the implanted tumor from the MHCC-97L cell line.
We successfully developed an orthotopic transplantation tumor model in nude mice with the use of an AGS. The success rate of tumor transplantation was improved from 60% in the control group to 100% in the experimental group using AGS. The detection of fluorescent signals showed that tumors grew in all live nude mice. The mice were divided into 3 groups: AGS-, AGS+/PAX5- and AGS+/PAX5+. Tumor size was significantly smaller in PAX5 transfected nude mice compared to control mice (P < 0.0001). These fluorescent signal results were consistent with observations made during surgery. Pathologic examination further confirmed that the tissues from the ectopic tumor were HCC. Results from RT-PCR proved that the HCC originated from MHCC-97L cells.
Using an AGS is a convenient and efficient way of establishing an indirect orthotopic liver transplantation tumor model with a high success rate.
为了提高同种异体移植在小鼠模型中的效果，我们使用可吸收明胶海绵（AGS）在裸鼠中建立了原位移植肿瘤模型。
将稳定表达荧光素酶基因的 MHCC-97L 肝癌（HCC）细胞注入裸鼠皮下区域。1 周后，取出异位肿瘤并移植到裸鼠左肝叶。使用 AGS 建立裸鼠原位移植肿瘤模型。转染抑癌基因配对盒基因 5（PAX5），验证模型。通过生物发光成像技术测量肿瘤生长情况。半定量逆转录聚合酶链反应（RT-PCR）和组织病理学用于确认来自 MHCC-97L 细胞系的植入肿瘤的致瘤性。
我们成功地使用 AGS 在裸鼠中开发了一种原位移植肿瘤模型。使用 AGS 后，肿瘤移植成功率从对照组的 60%提高到实验组的 100%。荧光信号检测表明所有活裸鼠中均有肿瘤生长。将小鼠分为 3 组：AGS-、AGS+/PAX5-和 AGS+/PAX5+。与对照组相比，转染 PAX5 的裸鼠肿瘤明显较小（P < 0.0001）。这些荧光信号结果与手术观察一致。病理检查进一步证实，异位肿瘤组织为 HCC。RT-PCR 结果证实 HCC 来源于 MHCC-97L 细胞。
使用 AGS 是一种方便、高效的建立高成功率间接原位肝移植肿瘤模型的方法。
