Service d'Ophtalmologie IV, Centre National d'Ophtalmologie des Quinze-Vingts, Paris, France.
Ocul Immunol Inflamm. 2013;21(1):61-8. doi: 10.3109/09273948.2012.730653.
To target the use of two biologic tests in the diagnostic of viral Herpesviridae anterior uveitis (AC) by the consideration of clinical behavior and delay of intraocular sampling.
Aqueous humor samples were collected from 42 patients suspected of having AU of infectious origin at presentation. The diagnosis of infectious uveitis was confirmed by quantification of antibodies with the Goldmann-Witmer coefficient (GWC) and/or detection of Herpesviridae genomes with PCR. The data were compared with data of 16 uveitis control samples used to calculate the specificity of the tests.
Sixteen out of 42 eyes (38%) had a final diagnosis of anterior segment infectious uveitis of viral origin (Herpesviridae) confirmed by PCR positive result (5/14 eyes; 14 of the 16 eyes were tested by PCR) and/or specific intraocular antibody synthesis (14/16 eyes).
While the GWC is progressively less often performed, these findings suggest that it still has a role in AU suspected of herpesvirus etiology.
通过考虑临床行为和眼内样本采集的延迟，针对两种生物检测在病毒性疱疹病毒前葡萄膜炎（AC）的诊断中的应用。
在就诊时，从 42 名疑似感染性 AU 的患者中采集房水样本。通过 Goldmann-Witmer 系数（GWC）定量检测抗体和/或 PCR 检测疱疹病毒基因组，确认感染性葡萄膜炎的诊断。将这些数据与 16 例葡萄膜炎对照样本的数据进行比较，以计算这些检测的特异性。
PCR 阳性结果（5/14 只眼；16 只眼中有 14 只眼进行了 PCR 检测）和/或特异性眼内抗体合成（14/16 只眼）证实，42 只眼中有 16 只眼（38%）最终诊断为疱疹病毒引起的前节感染性葡萄膜炎。
虽然 GWC 的应用越来越少，但这些发现表明，在疑似疱疹病毒病因的 AU 中，它仍然具有一定的作用。
