School of Biosciences and Biotechnology, University of Camerino , Camerino , Italy.
J Enzyme Inhib Med Chem. 2014 Feb;29(1):87-91. doi: 10.3109/14756366.2012.753883. Epub 2013 Jan 16.
Studies on the mechanisms of saliva secretion have indicated that carbonic anhydrase (CA) is expressed in mammalian salivary glands. The enzyme is present in the saliva as the only known secretory isoenzyme, CAVI; its activity has been related to the modulation of taste and caries development. Unlike mammals, in birds, saliva is produced by the so-called minor salivary glands, mostly concentrated in the tongue. The involvement of CA has never been explored in avian salivary secretion. Thus, we aimed here to ascertain the enzyme occurrence in the quail lingual glands by a parallel investigation of the distributional patterns of CA activity sites, as visualized by histochemistry, and the immunohistochemical patterns of cytosolic CAII and secretory CAVI. The comparative evaluation of our findings does not rule out that some CA isoforms, associated to basolateral borders of the secretory cells and antigenically different from cytosolic CAII and secretory CAVI, may be involved in the salivary secretion in the quail lingual glands.
唾液分泌机制的研究表明，碳酸酐酶（CA）在哺乳动物的唾液腺中表达。该酶以唯一已知的分泌同工酶 CAVI 的形式存在于唾液中；其活性与味觉调节和龋齿发展有关。与哺乳动物不同，鸟类的唾液由所谓的小唾液腺产生，主要集中在舌头上。CA 在鸟类唾液分泌中的作用从未被探索过。因此，我们旨在通过组织化学可视化的 CA 活性部位分布模式的平行研究来确定鹌鹑舌腺中该酶的存在，以及细胞质 CAII 和分泌性 CAVI 的免疫组织化学模式。我们研究结果的比较评估并不能排除某些 CA 同工酶与分泌细胞的基底外侧边界相关，并且与细胞质 CAII 和分泌性 CAVI 在抗原上不同，可能参与鹌鹑舌腺的唾液分泌。
