RhoA 信号转导活性与 ezrin 表达的关系及其对乳腺癌患者预后的意义。
Relationship of RhoA signaling activity with ezrin expression and its significance in the prognosis for breast cancer patients.
机构信息
Breast Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
出版信息
Chin Med J (Engl). 2013 Jan;126(2):242-7.
BACKGROUND
We have recently reported that RhoA may regulate the invasion and metastasis of breast cancer cells as an upstream signal of ezrin in vitro. In this study, we examined the relationship of RhoA signaling activity with ezrin expression in breast cancer and its prognostic significance in patients with breast cancer.
METHODS
Paraffin tumor sections of breast cancer were collected retrospectively from 487 patients diagnosed between 2001 and 2004. Immunohistochemical methods were used to detect the expression of RhoA, phosphorylated (activated) RhoA, and ezrin.
RESULTS
Ezrin overexpression was detectable in 15.2% of 487 invasive breast cancers. The majority (85.1%) of ezrin-overexpressing tumors coexpressed phosphorylated RhoA; 78.8% of tumors with phosphorylated RhoA cooverexpressed ezrin. Patients whose cancers showed overexpression of ezrin or expression of phosphorylated RhoA had shorter survival rates.
CONCLUSIONS
RhoA activation is important in human breast cancer due to its upregulation of ezrin; thus, agents that target phosphorylated RhoA may be useful in the treatment of tumors with ezrin overexpression.
背景
我们最近报道 RhoA 可能作为 ezrin 的上游信号，调节乳腺癌细胞的侵袭和转移。在本研究中，我们检测了 RhoA 信号活性与乳腺癌中 ezrin 表达的关系及其在乳腺癌患者中的预后意义。
方法
回顾性收集了 2001 年至 2004 年间诊断的 487 例浸润性乳腺癌患者的石蜡肿瘤切片。采用免疫组织化学方法检测 RhoA、磷酸化（激活）RhoA 和 ezrin 的表达。
结果
在 487 例浸润性乳腺癌中，可检测到 15.2%的 ezrin 过表达。大多数（85.1%）ezrin 过表达的肿瘤共同表达磷酸化 RhoA；78.8%的磷酸化 RhoA 共过表达 ezrin 的肿瘤。ezrin 过表达或磷酸化 RhoA 表达的患者生存率较低。
结论
由于 RhoA 上调 ezrin，RhoA 激活在人乳腺癌中很重要；因此，靶向磷酸化 RhoA 的药物可能对 ezrin 过表达的肿瘤的治疗有用。
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