Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
Chin Med J (Engl). 2013 Jan;126(2):296-9.
Programmed cell death 5 (PDCD5) is a novel apoptotic regulatory gene that promotes apoptosis in various tumor cells. Studies have shown that PDCD5 accelerates the apoptosis of synoviocytes in vitro, implying a potential role in rheumatoid arthritis (RA) pathogenesis. This study examined the expression of PDCD5 in serum and synovial fluid of RA patients, its effect on the expression of inflammatory cytokine, interleukin-17 (IL-17), and the assessment of disease activity in RA.
PDCD5 and IL-17 levels in serum and synovial fluid from 18 patients with RA and 22 patients with osteoarthritis (OA) were detected using enzyme-linked immunosorbent assay (ELISA). Concentrations of serum PDCD5 in 40 healthy people were also detected as controls. As disease activity indices, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), and X-ray grading scale were also evaluated.
Serum and synovial fluid PDCD5 levels in RA patients were significantly higher than those in OA and healthy controls. Serum PDCD5 level was inversely correlated to CRP and ESR, and was significantly higher in the RF negative group than in the positive group. PDCD5 level was also negatively correlated with IL-17 levels both in serum and synovial fluid of RA patients. However, differences in synovial fluid PDCD5 level from RA patients at different Larsen stages were not detectable.
PDCD5 affects RA pathogenesis. Insufficient apoptosis of fibroblast-like synoviocytes and inflammatory cells in RA could increase the expression of PDCD5 protein. As PDCD5 levels correlated negatively with disease activity indices and IL-17 level, PDCD5 could become a target in the diagnosis and treatment of RA.
程序性细胞死亡因子 5（PDCD5）是一种新的凋亡调控基因，可促进多种肿瘤细胞的凋亡。研究表明，PDCD5 可加速体外滑膜细胞的凋亡，提示其在类风湿关节炎（RA）发病机制中可能具有潜在作用。本研究检测了 PDCD5 在 RA 患者血清和滑液中的表达及其对炎症细胞因子白细胞介素-17（IL-17）表达的影响，并评估了其在 RA 中的疾病活动度。
采用酶联免疫吸附试验（ELISA）检测 18 例 RA 患者和 22 例骨关节炎（OA）患者血清和滑液中 PDCD5 和 IL-17 的水平，并检测 40 名健康人血清 PDCD5 浓度作为对照。同时评估 C 反应蛋白（CRP）、红细胞沉降率（ESR）、类风湿因子（RF）和 X 射线分级等疾病活动指标。
RA 患者血清和滑液 PDCD5 水平明显高于 OA 患者和健康对照组。血清 PDCD5 水平与 CRP 和 ESR 呈负相关，且 RF 阴性组明显高于阳性组。PDCD5 水平与 RA 患者血清和滑液中 IL-17 水平呈负相关。然而，不同 Larsen 分期 RA 患者滑液 PDCD5 水平的差异无统计学意义。
PDCD5 影响 RA 的发病机制。RA 中纤维母细胞样滑膜细胞和炎症细胞凋亡不足可能会增加 PDCD5 蛋白的表达。由于 PDCD5 水平与疾病活动指标和 IL-17 水平呈负相关，因此 PDCD5 可能成为 RA 诊断和治疗的新靶点。
