Has Cristina, Sitaru Cassian
Department of Molecular Dermatology, University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2013;961:1-16. doi: 10.1007/978-1-62703-227-8_1.
Groundbreaking advances on the molecular and cellular physiological and physiopathological skin processes, including the complete sequencing of the genome of several species and the increased availability of gene-modified organisms, paved the way to firmly establishing molecular approaches and methods in experimental, translational, and clinical dermatology. As a result, newly developed experimental ex vivo assays and animal models prove exquisite tools for addressing fundamental physiological cutaneous processes and pathogenic mechanisms of skin diseases. A plethora of new findings that were generated using these experimental tools serve as a strong basis for intense translational research efforts aiming at developing new, specific, and sensitive diagnostic tests and efficient "personalized" therapies with less side-effects. Consequently, a broad array of molecular diagnostic tests and therapies for a wide spectrum of skin diseases ranging from genodermatoses through skin neoplasms, allergy, inflammatory and autoimmune diseases, are already routinely used in the clinical dermatology practice. This article highlights several major developments in molecular experimental and clinical dermatology.
在分子和细胞生理及皮肤病理生理过程方面取得的突破性进展，包括多个物种基因组的完整测序以及基因修饰生物体的增多，为在实验性、转化性和临床皮肤病学中牢固确立分子方法和手段铺平了道路。因此，新开发的实验性体外检测方法和动物模型证明是研究皮肤基本生理过程和皮肤病致病机制的绝佳工具。利用这些实验工具产生的大量新发现，为旨在开发新的、特异性和敏感性诊断测试以及副作用较小的高效“个性化”疗法的深入转化研究工作奠定了坚实基础。因此，从遗传性皮肤病到皮肤肿瘤、过敏、炎症和自身免疫性疾病等广泛皮肤病的一系列分子诊断测试和疗法，已在临床皮肤病实践中常规使用。本文重点介绍分子实验性和临床皮肤病学的几项重大进展。
