Tsuruta Daisuke, Dainichi Teruki, Hamada Takahiro, Ishii Norito, Hashimoto Takashi
Department of Dermatology, School of Medicine, and Institute of Cutaneous Cell Biology, Kurume University, Kurume, Fukuoka, Japan.
Methods Mol Biol. 2013;961:17-32. doi: 10.1007/978-1-62703-227-8_2.
Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1-3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purified proteins from cell cultures and recombinant proteins.
自身免疫性大疱性疾病是特征最为明确的自身免疫性皮肤病。在过去三十年中，由于其靶自身抗原的鉴定，这些疾病的分子诊断已成为可能。在本综述中，我们总结了通过使用正常人皮肤切片、大鼠膀胱切片以及用桥粒芯胶蛋白1-3编码载体转染的COS7细胞，采用直接和间接免疫荧光技术相结合的方法，对自身免疫性大疱性疾病进行分类的方法，以及使用正常人表皮提取物、真皮提取物、细胞培养物中的纯化蛋白和重组蛋白进行酶联免疫吸附测定和免疫印迹的方法。
