MA 和 NC 结构域的改变调节劳斯肉瘤病毒 Gag 蛋白依赖于磷酸肌醇的质膜定位。
Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus Gag protein.
机构信息
Department of Medicine, Penn State College of Medicine, Hershey, PA, USA.
出版信息
J Virol. 2013 Mar;87(6):3609-15. doi: 10.1128/JVI.03059-12. Epub 2013 Jan 16.
Abstract
Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P(2)] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P(2) and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P(3)] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P(2) and PI(3,4,5)P(3), suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.
摘要
逆转录病毒 Gag 蛋白指导病毒粒子从质膜（PM）组装。磷脂酰肌醇-(4,5)-二磷酸 [PI(4,5)P(2)] 在几种逆转录病毒 Gag 蛋白的 PM 靶向中起作用。在这里，我们报告细胞内 PI(4,5)P(2) 和磷脂酰肌醇-(3,4,5)-三磷酸 [PI(3,4,5)P(3)] 水平的耗竭会损害 Rous 肉瘤病毒 (RSV) Gag PM 定位。核运输缺陷的 Gag 突变体对细胞内 PI(4,5)P(2) 和 PI(3,4,5)P(3) 的减少不太敏感，这表明 Gag 核运输和磷酸肌醇依赖性 PM 靶向之间可能存在联系。
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