Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA.
Mol Cell Proteomics. 2013 Apr;12(4):874-84. doi: 10.1074/mcp.R112.026310. Epub 2013 Jan 16.
The correlations between protein glycosylation and many biological processes and diseases are increasing the demand for quantitative glycomics strategies enabling sensitive monitoring of changes in the abundance and structure of glycans. This is currently attained through multiple strategies employing several analytical techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. The detection and quantification of glycans often involve labeling with ionic and/or hydrophobic reagents. This step is needed in order to enhance detection in spectroscopic and mass spectrometric measurements. Recently, labeling with stable isotopic reagents has also been presented as a very viable strategy enabling relative quantitation. The different strategies available for reliable and sensitive quantitative glycomics are herein described and discussed.
蛋白质糖基化与许多生物过程和疾病的相关性正在增加，这就需要定量糖组学策略来实现对聚糖丰度和结构变化的敏感监测。目前，这是通过采用多种分析技术（如毛细管电泳、液相色谱和质谱）的多种策略来实现的。糖链的检测和定量通常涉及与离子和/或疏水性试剂标记。这一步骤是为了增强光谱和质谱测量中的检测。最近，稳定同位素试剂的标记也被提出作为一种非常可行的策略，能够实现相对定量。本文描述并讨论了用于可靠和灵敏定量糖组学的不同策略。
