坦桑尼亚桑给巴尔群岛高产霍乱毒素埃尔托变体霍乱弧菌菌株的分子特征。
Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania.
机构信息
National Institute of Cholera and Enteric Diseases, Kolkata, India.
出版信息
J Clin Microbiol. 2013 Mar;51(3):1040-5. doi: 10.1128/JCM.03162-12. Epub 2013 Jan 16.
Abstract
Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.
摘要
2009 年，对桑给巴尔的 1180 份腹泻粪便样本进行分析，发现了 247 株霍乱弧菌 O1 型，稻叶型。表型特征和基于 PCR 的 rstR、rtxC 和 tcpA 等位基因检测表明，它们属于 El Tor 生物型。这些菌株 ctxB 的遗传分析表明它们是经典型，并且通过 Western blot 证实了经典霍乱毒素 B（CTB）的产生。这些菌株产生的 CT 比原型 El Tor 多，并通过脉冲场凝胶电泳（PFGE）分析形成了一个单独的簇。
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