National Institute of Cholera and Enteric Diseases, Kolkata, India.
J Clin Microbiol. 2012 May;50(5):1733-6. doi: 10.1128/JCM.00387-12. Epub 2012 Feb 22.
A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of ctxB alleles. Our retrospective study using this newly developed PCR showed that Haitian ctxB first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant strains isolated recently that was distinct from the PFGE pattern of the strains carrying classical ctxB that closely matched the 2006 to 2007 variant strains.
建立了一种基于 PCR 的方法来区分经典、El Tor 和海地型 ctxB 等位基因。我们使用新开发的 PCR 进行的回顾性研究表明,海地 ctxB 于 2006 年 4 月首次出现在加尔各答,2011 年分离的菌株中有 93.3%携带新等位基因。聚类分析显示,最近分离的新型菌株的脉冲场凝胶电泳(PFGE)模式与携带经典 ctxB 的菌株的 PFGE 模式不同,与 2006 年至 2007 年的变异株密切匹配。