应用基于实时 PCR 的检测方法评估 Epstein-Barr 病毒、人类疱疹病毒 6（HHV-6）和 HHV-8 抗病毒药物敏感性。
Evaluation of Epstein-Barr virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays.
机构信息
Infectious Disease Research Centre, Laval University, Quebec City, Quebec, Canada.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1244-6. doi: 10.1128/JCM.03234-12. Epub 2013 Jan 16.
Abstract
Evaluation of candidate antiviral drugs against Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), and HHV-8 is hampered by the lack of convenient laboratory assays. We developed real-time quantitative PCR assays performed on supernatants of lymphoma cell lines and determined the 50% inhibitory concentrations (IC50s) of nucleoside, nucleotide, and pyrophosphate analogues against these herpesviruses.
摘要
评估候选抗病毒药物对 Epstein-Barr 病毒 (EBV)、人类疱疹病毒 6 (HHV-6) 和 HHV-8 的效果受到缺乏方便的实验室检测方法的限制。我们开发了在淋巴瘤细胞系上清液中进行的实时定量 PCR 检测方法，并确定了核苷、核苷酸和焦磷酸类似物对这些疱疹病毒的 50%抑制浓度 (IC50)。
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Evaluation of Epstein-Barr virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays.应用基于实时 PCR 的检测方法评估 Epstein-Barr 病毒、人类疱疹病毒 6（HHV-6）和 HHV-8 抗病毒药物敏感性。
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