实时聚合酶链反应作为研究人疱疹病毒6对抗病毒药物敏感性的通用工具。
Real-time PCR as a versatile tool for investigating the susceptibility of human herpesvirus 6 to antiviral agents.
作者信息
Macé Muriel, Manichanh Chaysavanh, Bonnafous Pascale, Précigout Stéphanie, Boutolleau David, Gautheret-Dejean Agnès, Agut Henri
机构信息
Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.
出版信息
Antimicrob Agents Chemother. 2003 Sep;47(9):3021-4. doi: 10.1128/AAC.47.9.3021-3024.2003.
Abstract
A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.
摘要
开发了一种定量实时PCR检测方法，用于测定人疱疹病毒6的抗病毒药物敏感性和生长动力学。然后对敏感株HST及其更昔洛韦耐药衍生物GCVR1的敏感性和适应性进行了表征，从而使我们得出结论，后一种病毒的突变并未显著改变其适应性。
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