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Real-time PCR as a versatile tool for investigating the susceptibility of human herpesvirus 6 to antiviral agents.实时聚合酶链反应作为研究人疱疹病毒6对抗病毒药物敏感性的通用工具。
Antimicrob Agents Chemother. 2003 Sep;47(9):3021-4. doi: 10.1128/AAC.47.9.3021-3024.2003.
2
Real-time PCR determination of human herpesvirus 6 antiviral drug susceptibility.人疱疹病毒6型抗病毒药物敏感性的实时聚合酶链反应测定
J Virol Methods. 2007 Mar;140(1-2):25-31. doi: 10.1016/j.jviromet.2006.10.009. Epub 2006 Dec 6.
3
Susceptibility of human herpesvirus 6 to antiviral compounds by flow cytometry analysis.通过流式细胞术分析人疱疹病毒6对抗病毒化合物的敏感性。
Cytometry. 2000 Jun 1;40(2):135-40. doi: 10.1002/(sici)1097-0320(20000601)40:2<135::aid-cyto7>3.0.co;2-h.
4
Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with the death of an allogeneic stem cell transplant recipient.具有与一名异基因干细胞移植受者死亡相关的氨基酸替代的人疱疹病毒6型更昔洛韦耐药株。
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Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus.实时聚合酶链反应在单纯疱疹病毒抗病毒药物敏感性测定中的应用
Antimicrob Agents Chemother. 2002 Sep;46(9):2943-7. doi: 10.1128/AAC.46.9.2943-2947.2002.
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J Infect Dis. 1990 Sep;162(3):634-7. doi: 10.1093/infdis/162.3.634.
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Evaluation of Epstein-Barr virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays.应用基于实时 PCR 的检测方法评估 Epstein-Barr 病毒、人类疱疹病毒 6（HHV-6）和 HHV-8 抗病毒药物敏感性。
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Reply to "Calibration technologies for correct determination of Epstein-Barr Virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays".对“通过基于实时PCR的检测方法正确测定爱泼斯坦-巴尔病毒、人类疱疹病毒6型（HHV-6）和HHV-8抗病毒药物敏感性的校准技术”的回复
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2
Evaluation of Epstein-Barr virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays.应用基于实时 PCR 的检测方法评估 Epstein-Barr 病毒、人类疱疹病毒 6（HHV-6）和 HHV-8 抗病毒药物敏感性。
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3
Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay.通过多重实时聚合酶链反应法同时定量检测移植受者样本中的爱泼斯坦-巴尔病毒、巨细胞病毒和人疱疹病毒6型DNA。
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4
Ether lipid ester derivatives of cidofovir inhibit polyomavirus BK replication in vitro.西多福韦的醚脂酯衍生物在体外抑制多瘤病毒BK复制。
Antimicrob Agents Chemother. 2006 Apr;50(4):1564-6. doi: 10.1128/AAC.50.4.1564-1566.2006.

本文引用的文献

1
Human herpesvirus (HHV)-6 and HHV-7: two closely related viruses with different infection profiles in stem cell transplantation recipients.人疱疹病毒（HHV）-6和HHV-7：两种密切相关的病毒，在干细胞移植受者中具有不同的感染特征。
J Infect Dis. 2003 Jan 15;187(2):179-86. doi: 10.1086/367677. Epub 2003 Jan 6.
2
Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus.实时聚合酶链反应在单纯疱疹病毒抗病毒药物敏感性测定中的应用
Antimicrob Agents Chemother. 2002 Sep;46(9):2943-7. doi: 10.1128/AAC.46.9.2943-2947.2002.
3
Development of a real-time polymerase chain reaction assay for the diagnosis of human herpesvirus-6 infection and application to bone marrow transplant patients.
J Virol Methods. 2002 Feb;100(1-2):27-35. doi: 10.1016/s0166-0934(01)00390-1.
4
Selection of the same mutation in the U69 protein kinase gene of human herpesvirus-6 after prolonged exposure to ganciclovir in vitro and in vivo.在体外和体内长时间暴露于更昔洛韦后，人疱疹病毒6型U69蛋白激酶基因中相同突变的选择。
J Gen Virol. 2001 Nov;82(Pt 11):2767-2776. doi: 10.1099/0022-1317-82-11-2767.
5
(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays showed a lack of toxicity of ganciclovir (GCV), C.OXTs, and their derivatives, to TaY(OK) cells at 1 microM. Therefore we compared the antiviral potencies of these drugs at 1 microM by monitoring the viral loads produced during a 1-day period during the course of the drug treatment. Among the drugs tested, 3'-fluorocarbocyclic oxetanocin A (3'F-C.OXT-A) was the most effective for inhibiting the virus production, and at concentrations ranging from 0.5 microM to 10 microM, the inhibition of the viral production was dose-dependent. A comparison of the chemical structures of the derivatives with that of C.OXT-A, which is the parental molecule, suggested that the 3'-fluorine-modification might account for the higher anti-HHV-6 activity and lower cytotoxicity.
Microbiol Immunol. 2001;45(6):457-66. doi: 10.1111/j.1348-0421.2001.tb02645.x.
6
Susceptibility of human herpesvirus 6 to antiviral compounds by flow cytometry analysis.通过流式细胞术分析人疱疹病毒6对抗病毒化合物的敏感性。
Cytometry. 2000 Jun 1;40(2):135-40. doi: 10.1002/(sici)1097-0320(20000601)40:2<135::aid-cyto7>3.0.co;2-h.
7
Frequent HHV-6 reactivation in multiple sclerosis (MS) and chronic fatigue syndrome (CFS) patients.多发性硬化症（MS）和慢性疲劳综合征（CFS）患者中HHV-6频繁再激活。
J Clin Virol. 2000 May;16(3):179-91. doi: 10.1016/s1386-6532(99)00079-7.
8
Selection and characterization of two specific monoclonal antibodies directed against the two variants of human herpesvirus-6.
Res Virol. 1998 Nov-Dec;149(6):403-11. doi: 10.1016/s0923-2516(99)80008-9.
9
Cytomegalovirus and human herpesvirus 6 both cause viral disease after renal transplantation.
Transplantation. 1998 Oct 15;66(7):877-82. doi: 10.1097/00007890-199810150-00011.
10
Antiviral activity of selected acyclic nucleoside analogues against human herpesvirus 6.所选无环核苷类似物对人疱疹病毒6的抗病毒活性。
Antiviral Res. 1995 Dec;28(4):343-57. doi: 10.1016/0166-3542(95)00058-5.

实时聚合酶链反应作为研究人疱疹病毒6对抗病毒药物敏感性的通用工具。

Real-time PCR as a versatile tool for investigating the susceptibility of human herpesvirus 6 to antiviral agents.

作者信息

Macé Muriel, Manichanh Chaysavanh, Bonnafous Pascale, Précigout Stéphanie, Boutolleau David, Gautheret-Dejean Agnès, Agut Henri

机构信息

Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.

出版信息

Antimicrob Agents Chemother. 2003 Sep;47(9):3021-4. doi: 10.1128/AAC.47.9.3021-3024.2003.

DOI:10.1128/AAC.47.9.3021-3024.2003
PMID:12937019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC182648/
Abstract

A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

摘要

开发了一种定量实时PCR检测方法，用于测定人疱疹病毒6的抗病毒药物敏感性和生长动力学。然后对敏感株HST及其更昔洛韦耐药衍生物GCVR1的敏感性和适应性进行了表征，从而使我们得出结论，后一种病毒的突变并未显著改变其适应性。