有证据表明，分离的肝细胞的质膜流动性会因暴露于微管解聚药物而发生改变。

Evidence that plasma membrane fluidity of isolated hepatocytes is modified by exposure to microtubule-depolymerizing drugs.

作者信息

Benedetti A, Marucci L, Ferretti G, Curatola G, Jézéquel A M, Orlandi F

机构信息

Institute of Experimental Pathology, University of Ancona, School of Medicine, Italy.

出版信息

J Hepatol. 1990 Mar;10(2):144-8. doi: 10.1016/0168-8278(90)90043-q.

DOI:10.1016/0168-8278(90)90043-q

PMID:2332583

Abstract

The role of microtubules on membrane fluidity has been investigated on freshly isolated whole rat hepatocytes prepared by the perfusion method and exposed either to the microtubule-depolymerizing drugs colchicine and vincristine or to beta-lumicolchicine, a colchicine analog deprived of biological activity. Exposure of hepatocytes to 6.3 microM colchicine or to 3.0 microM vincristine led to a significant decrease of membrane fluidity as measured by fluorescence polarization of trimethylammoniodiphenylhexatriene (TMA-DPH). No changes were observed in cells exposed to 10.0 microM beta-lumicolchicine. These observations support the hypothesis that the microtubular system plays a role in the modulations of physico-chemical properties of the plasma membrane.

摘要

通过灌注法制备的新鲜分离的大鼠全肝细胞，研究了微管对膜流动性的作用。这些肝细胞分别暴露于微管解聚药物秋水仙碱和长春新碱，或暴露于β-光秋水仙碱（一种无生物活性的秋水仙碱类似物）。用三甲基铵二苯基己三烯（TMA-DPH）的荧光偏振测量，肝细胞暴露于6.3微摩尔秋水仙碱或3.0微摩尔长春新碱会导致膜流动性显著降低。暴露于10.0微摩尔β-光秋水仙碱的细胞未观察到变化。这些观察结果支持了微管系统在调节质膜物理化学性质中起作用的假说。