大鼠离体肝细胞质膜流动性的年龄与性别相关变化

Age and sex related changes of plasma membrane fluidity in isolated rat hepatocytes.

作者信息

Benedetti A, Ferretti G, Curatola G, Jézéquel A M, Orlandi F

机构信息

Postgraduate School of Gastroenterology, University of Ancona, School of Medicine, Italy.

出版信息

Biochem Biophys Res Commun. 1988 Oct 31;156(2):840-5. doi: 10.1016/s0006-291x(88)80920-3.

DOI:10.1016/s0006-291x(88)80920-3

PMID:3190683

Abstract

The influence of sex and age on membrane fluidity, has been investigated in 6, 12, 18 weeks old Sprague-Dawley rats. Fluorescence polarization (P) was determined at 37 degrees C with a Perkin Elmer MPF 44A fluorescence spectrophotometer. The fluorescent probe TMA-DPH was added to isolated hepatocytes prepared by collagenase method. The membrane fluidity was constantly lower in males than in females, but the difference was statistically significant only in the 12 weeks old group. Major differences appeared related to aging with a significant age-related decrease in fluidity in all animals.

摘要

在6周龄、12周龄和18周龄的斯普拉格-道利大鼠中，研究了性别和年龄对膜流动性的影响。使用珀金埃尔默MPF 44A荧光分光光度计在37摄氏度下测定荧光偏振（P）。将荧光探针TMA-DPH添加到通过胶原酶法制备的分离肝细胞中。雄性大鼠的膜流动性始终低于雌性大鼠，但仅在12周龄组中差异具有统计学意义。主要差异似乎与衰老有关，所有动物的膜流动性均随年龄增长而显著下降。