Goodnough M C, Johnson E A
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706.
Appl Environ Microbiol. 1992 Oct;58(10):3426-8. doi: 10.1128/aem.58.10.3426-3428.1992.
Botulinum toxin for medical use is diluted to very low concentrations (nanograms per milliliter); when it is preserved by lyophilization, considerable loss of activity can occur. In the present study, conditions that gave > 90% recovery of the toxicity after lyophilization of solutions containing 20 to 1,000 mouse 50% lethal doses per ml were found. Toxicity was recovered upon drying 0.1 ml of toxin solution when the pH was maintained below 7 and bovine or human serum albumins were used as stabilizers. Various other substances tested with albumin, including glucose, sucrose, trehalose, mannitol, glycine, and cellibiose, did not increase recovery on drying.
医用肉毒杆菌毒素被稀释到非常低的浓度（每毫升纳克）；当通过冻干保存时，可能会发生相当大的活性损失。在本研究中，发现了在冻干每毫升含有20至1000小鼠50%致死剂量的溶液后，毒性回收率>90%的条件。当pH值保持在7以下，并用牛血清白蛋白或人血清白蛋白作为稳定剂时，干燥0.1毫升毒素溶液后毒性得以恢复。与白蛋白一起测试的各种其他物质，包括葡萄糖、蔗糖、海藻糖、甘露醇、甘氨酸和纤维二糖，在干燥时并没有提高回收率。
