Linh Le Hung, Linh Ta Hong, Xuan Tran Dang, Ham Le Huy, Ismail Abdelbagi M, Khanh Tran Dang
Agricultural Genetics Institute, Tu Liem, Hanoi, Vietnam.
Int J Plant Genomics. 2012;2012:949038. doi: 10.1155/2012/949038. Epub 2012 Dec 27.
Rice is a stable food in Vietnam and plays a key role in the economy of the country. However, the production and the cultivating areas are adversely affected from the threats of devastation caused by the rise of sea level. Using marker-assisted backcrossing (MABC) to develop a new salt tolerance rice cultivar is one of the feasible methods to cope with these devastating changes. To improve rice salt tolerance in BT7 cultivar, FL478 was used as a donor parent to introgress the Saltol QTL conferring salt tolerance into BT7. Three backcrosses were conducted and successfully transferred positive alleles of Saltol from FL478 into BT7. The plants numbers IL-30 and IL-32 in BC(3)F(1) population expected recurrent genome recovery of up to 99.2% and 100%, respectively. These selected lines that carried the Saltol alleles were screened in field for their agronomic traits. All improved lines had Saltol allele similar to the donor parent FL478, whereas their agronomic performances were the same as the original BT7. We show here the success of improving rice salt tolerance by MABC and the high efficiency of selection in early generations. In the present study, MABC has accelerated the development of superior qualities in the genetic background of BT7.
水稻是越南的主食，在该国经济中发挥着关键作用。然而，海平面上升造成的破坏威胁对水稻产量和种植面积产生了不利影响。利用标记辅助回交（MABC）培育新的耐盐水稻品种是应对这些破坏性变化的可行方法之一。为提高BT7品种水稻的耐盐性，以FL478作为供体亲本，将赋予耐盐性的Saltol QTL导入BT7。进行了三次回交，并成功地将FL478的Saltol正向等位基因转移到BT7中。BC(3)F(1)群体中的IL-30和IL-32植株预计轮回基因组恢复率分别高达99.2%和100%。对这些携带Saltol等位基因的入选株系进行田间农艺性状筛选。所有改良株系都具有与供体亲本FL478相似的Saltol等位基因，而它们的农艺表现与原始BT7相同。我们在此展示了通过MABC提高水稻耐盐性的成功以及早期世代选择的高效率。在本研究中，MABC加速了BT7遗传背景下优良品质的培育。
