Kim Tae-Heon, Kim Suk-Man
Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Ecological & Environmental System, Kyungpook National University, Sangju 37224, Republic of Korea.
Plants (Basel). 2023 Mar 21;12(6):1401. doi: 10.3390/plants12061401.
Salt stress is a major constraint in rice production worldwide. Salt stress is estimated to cause annual losses of 30-50% in rice production. Discovering and deploying salt-resistance genes are the most effective ways to control salt stress. We performed a genome-wide association study (GWAS) to detect QTLs related to salt tolerance at the seedling stage using the -multiparent advanced generation intercross (MAGIC) population. Four QTLs (, , , and ) associated with salt tolerance were identified on chromosomes 1, 2, and 9. Among these QTLs, a novel QTL, , was located between flanking SNPs (1354576 and id1028360) on chromosome 1, with the largest -log10(P) value of 5.81 and a total phenotypic variance of 15.2%. RNA-seq analysis revealed that among the seven differentially expressed genes (DEGs) commonly identified in both P6 and JM298 showing salt tolerance, two upregulated genes, (ASR transcription factor) and (), related to salt and drought tolerance, were also involved in the target region of . The results of this study can provide insights into further understanding of salt tolerance mechanisms and developing DNA markers for marker-assisted selection (MAS) breeding to improve the salt tolerance of cultivars in rice breeding programs.
盐胁迫是全球水稻生产的主要限制因素。据估计，盐胁迫每年导致水稻产量损失30%-50%。发现和利用耐盐基因是控制盐胁迫的最有效方法。我们利用多亲本高世代杂交（MAGIC）群体进行了全基因组关联研究（GWAS），以检测与苗期耐盐性相关的QTL。在第1、2和9号染色体上鉴定出4个与耐盐性相关的QTL（、、和）。在这些QTL中，一个新的QTL位于第1号染色体上侧翼SNP（1354576和id1028360）之间，-log10(P)值最大为5.81，总表型变异为15.2%。RNA-seq分析显示，在P6和JM298这两个表现出耐盐性的材料中共同鉴定出的7个差异表达基因（DEG）中，两个上调基因（ASR转录因子）和（）与耐盐和耐旱相关，也位于的目标区域内。本研究结果可为进一步了解耐盐机制以及开发DNA标记用于标记辅助选择（MAS）育种提供参考，以提高水稻育种计划中品种的耐盐性。
