Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
PLoS One. 2013;8(1):e53710. doi: 10.1371/journal.pone.0053710. Epub 2013 Jan 10.
The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.
五跨膜蛋白 CD133（也称为 prominin-1）已被广泛用作癌症和正常干细胞的标志物。然而，CD133 的功能尚未阐明。在这里，我们描述了一种从卵巢透明细胞癌 (CCC) 中建立的癌症干细胞系，并表明 CD133 与桥粒连接蛋白（也称为 γ-连环蛋白）相互作用，桥粒连接蛋白是一种桥粒连接蛋白。我们进一步证明，通过 RNA 干扰 (RNAi) 敲低 CD133 会导致桥粒钙粘蛋白 desmoglein-2 的下调，并破坏 CCC 干细胞的细胞间黏附性和致瘤性。我们推测 CD133 可能是癌症化疗的一个有前途的靶点。
