嚼烟使用者口腔颊黏膜上皮细胞的微核检测
Micronucleus investigation in human buccal epithelial cells of gutkha users.
作者信息
Jyoti Smita, Khan Saif, Afzal Mohammad, Siddique Yasir Hasan
机构信息
Department of Zoology, Human Genetics and Toxicology Laboratory, Section of Genetics, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
出版信息
Adv Biomed Res. 2012;1:35. doi: 10.4103/2277-9175.100128. Epub 2012 Aug 28.
BACKGROUND
Gutkha is a cheap and convenient betel quid substitute, which is popular among all age groups. Various studies reveal its carcinogenic nature that leads to oral submucosus fibrosis and increases the chances of oral cancer. The micronucleus (MN) assay in exfoliated mucosal cells is a useful method for observing genetic damage in humans.
AIM
To observe the genotoxic effect of gutkha on human buccal epithelial cells.
MATERIALS AND METHODS
The MN assay was performed to assess the frequency of MN in human buccal epithelial cells. The study comprises 60 individuals of which 30 individuals were gutkha chewers and another 30 were nonusers (control). The MN frequency was scored to estimate the genotoxic damage.
RESULTS
In gutkha users, the frequency of MN was highly significant (17.4 ± 0.944) as compared with nonusers (control) groups (4.53 ± 0.331) (P < 0.001).
CONCLUSIONS
The MN assay in human buccal epithelial cells is a useful and minimally invasive method for monitoring genetic damage in humans. Asignificantly higher frequency of micronucleated cells are found among gutkha users.
背景
古特卡是一种廉价且方便的槟榔替代品，在各年龄组中都很受欢迎。各种研究揭示了其致癌性质，会导致口腔黏膜下纤维化并增加患口腔癌的几率。脱落黏膜细胞中的微核（MN）检测是观察人类遗传损伤的一种有用方法。
目的
观察古特卡对人颊黏膜上皮细胞的遗传毒性作用。
材料与方法
采用MN检测评估人颊黏膜上皮细胞中MN的频率。该研究包括60名个体，其中30名是古特卡咀嚼者，另外30名是非使用者（对照组）。对MN频率进行评分以估计遗传毒性损伤。
结果
与非使用者（对照组）（4.53±0.331）相比，古特卡使用者的MN频率非常显著（17.4±0.944）（P<0.001）。
结论
人颊黏膜上皮细胞中的MN检测是监测人类遗传损伤的一种有用且微创的方法。在古特卡使用者中发现微核细胞的频率显著更高。
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