Arul P, Shetty Smitha, Masilamani Suresh, Akshatha C, Naveen Kumar B J
Department of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Perambalur, Tamil Nadu, India.
Indian J Med Paediatr Oncol. 2017 Jul-Sep;38(3):273-276. doi: 10.4103/ijmpo.ijmpo_26_16.
Liquid-based cytology (LBC) can be used for the evaluation of micronucleus (MN) in exfoliated buccal epithelial cells of populations occupationally exposed to potentially carcinogenic agents.
This study was undertaken to evaluate the frequency of MN of exfoliated buccal epithelial cells using LBC preparation in petrol station workers.
Fifty petrol station workers (cases) and fifty hospital administrative staffs (controls) were recruited and evaluated for MN by May-Grunwald Giemsa, Hematoxylin and Eosin, and Papanicolaou stains using LBC preparation. Statistical analysis was performed with Student's -test, and < 0.05 was considered statistically significant.
Regardless of staining method used, the mean frequency of MN for cases was significantly higher than that of controls ( < 0.001).
The present study concluded that petrol station workers are under risk of significant cytogenetic damage. The MN in exfoliated buccal epithelial cells found to be a useful biomarker of occupational exposure to genotoxic chemicals. LBC can be used for sample preparation to evaluate the frequency of MN in those who are occupationally exposed to potentially carcinogenic agents in view of overall improvement on sample preservation and visualization of cell morphology.
液基细胞学（LBC）可用于评估职业暴露于潜在致癌剂人群的颊部脱落上皮细胞中的微核（MN）。
本研究旨在使用LBC制片法评估加油站工作人员颊部脱落上皮细胞的微核频率。
招募50名加油站工作人员（病例组）和50名医院行政人员（对照组），使用LBC制片法通过May-Grunwald Giemsa、苏木精和伊红以及巴氏染色对微核进行评估。采用Student's t检验进行统计分析，P<0.05被认为具有统计学意义。
无论使用何种染色方法，病例组的微核平均频率均显著高于对照组（P<0.001）。
本研究得出结论，加油站工作人员存在显著细胞遗传学损伤风险。颊部脱落上皮细胞中的微核被发现是职业性接触遗传毒性化学物质的有用生物标志物。鉴于样本保存和细胞形态可视化的整体改善，LBC可用于样本制备以评估职业暴露于潜在致癌剂人群的微核频率。
