Masoko Peter, Picard Jackie, Eloff Jacobus N
Phytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa.
Onderstepoort J Vet Res. 2010 Oct 1;77(1):E1-7. doi: 10.4102/ojvr.v77i1.2.
Wound healing is a fundamental response to tissue injury and several natural products have been shown to accelerate the healing process. The present study was undertaken to determine the safety and efficacy of the topical treatment of acetone leaf extracts of Combretum imberbe, Combretum nelsonii,Combretum albopuntactum and Terminalia sericea based on their in vitro antimicrobial activity. Four circular full-thickness skin wounds were made on the backs of eight anaesthetised Wistar rats using aseptic techniques. The treatments were administrated topically using 10% and 20% concentrations of each extract in aqueous cream in separate treatments. Indications of erythema, exudate, crust formation,swelling and ulceration were used to determine the wound healing process. All of the wounds closed completely within 17 days. Throughout the experiment, a subcutaneous probe was used to determine that the body temperature and body weight of the rats were within the normal range. C. imberbe and C. nelsonii extracts accelerated wound healing, but there was no significant difference in wound contraction using 10% and 20% concentrations of the extracts in cream. The results also showed the potential usefulness of this model to measure accelerating wound healing.The extracts could perhaps overcome defects associated with healing failure in chronic wounds and prevent secondary bacterial and fungal infections.
伤口愈合是对组织损伤的一种基本反应，已有多种天然产物被证明可加速愈合过程。本研究旨在基于其体外抗菌活性，确定对南非风车子、尼尔森风车子、白刺风车子和绢毛榄仁树丙酮叶提取物进行局部治疗的安全性和有效性。采用无菌技术在8只麻醉的Wistar大鼠背部制作4个圆形全层皮肤伤口。分别使用每种提取物在水性乳膏中10%和20%的浓度进行局部治疗。通过观察红斑、渗出物、结痂、肿胀和溃疡情况来确定伤口愈合过程。所有伤口均在17天内完全愈合。在整个实验过程中，使用皮下探针确定大鼠的体温和体重在正常范围内。南非风车子和尼尔森风车子提取物加速了伤口愈合，但在乳膏中使用10%和20%浓度的提取物时，伤口收缩方面没有显著差异。结果还表明该模型在测量加速伤口愈合方面具有潜在用途。这些提取物或许可以克服与慢性伤口愈合失败相关的缺陷，并预防继发性细菌和真菌感染。
