Olaifa Folashade, Ayo Joseph O, Ambali Suleiman F, Rekwot Peter I
Department of Physiology and Pharmacology, Ahmadu Bello University.
Onderstepoort J Vet Res. 2012 Dec 5;79(1):E1-5. doi: 10.4102/ojvr.v79i1.413.
Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.
进行实验的目的是研究包装对驴红细胞渗透脆性（EOF）和丙二醛（MDA）浓度的影响，以及抗坏血酸（AA）的作用。十二头在传统粗放饲养系统下饲养的看似健康的驴作为实验对象。六头口服AA（200毫克/千克）并进行包装的驴用作实验动物，另外六头未给予AA的驴用作对照。在包装前后从所有驴采集血样以测定MDA和EOF。在0.3%氯化钠（NaCl）浓度下，对照驴的溶血百分比为93.69%±2.21%，该值显著高于（P<0.05）实验驴记录的71.31%±8.33%的值。对照驴包装后获得的MDA浓度为39.62微摩尔±4.16微摩尔，与实验驴记录的35.97微摩尔±2.88微摩尔的值无显著差异（P>0.05）。总之，驴溶血增加表明包装诱导了氧化应激，而给予AA可改善这种情况。
