The Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, the First Affiliated Hospital, Soochow University, Suzhou 215123, People's Republic of China.
Clin Sci (Lond). 2013 Jun;124(12):709-17. doi: 10.1042/CS20120517.
Corin is a cardiac protease that regulates BP (blood pressure) by activating natriuretic peptides. Recent animal studies identified corin expression in the kidney where it may regulate renal function. In the present study, we tested the hypothesis that corin may be present in human urine and that urinary corin levels may be altered in patients with kidney disease. We obtained urine and kidney tissue samples from normal individuals and CKD (chronic kidney disease) patients. Using ELISA, we detected corin protein in human urine. In normal individuals, urinary corin levels did not correlate with that of plasma, indicating that urinary corin is probably of kidney origin. Compared with normal controls, CKD patients had markedly reduced urinary corin levels and this reduction correlated with disease severity. By immunostaining, human corin protein was identified on the epithelial cell surface in renal tubules. The renal corin mRNA and protein levels were significantly lower in CKD patients than non-CKD controls. The results indicate that renal tubular corin may be shed into urine and that urinary and renal corin levels were reduced in CKD patients. These data suggest that reduced corin levels in the kidney may reflect the underlying pathology in CKD.
卡里安是一种心脏蛋白水解酶，通过激活利钠肽来调节血压（血压）。最近的动物研究表明，卡里安在肾脏中有表达，可能在调节肾功能方面发挥作用。在本研究中，我们检验了这样一个假设，即卡里安可能存在于人类尿液中，并且肾脏疾病患者的尿液卡里安水平可能会发生改变。我们从正常个体和 CKD（慢性肾脏疾病）患者中获得了尿液和肾脏组织样本。使用 ELISA，我们检测到了人尿液中的卡里安蛋白。在正常个体中，尿中卡里安水平与血浆中无相关性，表明尿中卡里安可能来源于肾脏。与正常对照组相比，CKD 患者的尿中卡里安水平显著降低，且这种降低与疾病严重程度相关。通过免疫染色，在肾小管的上皮细胞表面鉴定出人类卡里安蛋白。CKD 患者的肾中卡里安 mRNA 和蛋白水平明显低于非 CKD 对照组。这些结果表明，肾小管中的卡里安可能被分泌到尿液中，并且 CKD 患者的尿中和肾中的卡里安水平降低。这些数据表明，肾脏中卡里安水平的降低可能反映了 CKD 的潜在病理。
