Sun Yue-feng, Wu Yi-dong, Wu Lei, Jiang Juan-juan, Gao Rong, Xu Bin, Chen Xiao-wei, Zhao Zheng-yan
Department of Otorhinolaryngology Head and Neck Surgery, The Children Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Genetics (Zhejiang Unversity), Ministry of Education, Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou 310003, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Dec;47(12):974-7.
The purpose of this prospective study was to investigate the presence of human papillomavirus (HPV) in tonsillectomy and adenoidectomy specimens from pediatric patients without juvenile-onset recurrent respiratory papillomatosis (JORRP), so as to understand the effect of HPV infection in the upper respiratory tract in children.
Two hundred and forty-one pediatric patients without known JORRP or other HPV-related diseases undergoing tonsillectomy and/or adenoidectomy for hypertrophy or chronic tonsillitis were enrolled in this prospective study. One hundred and seventy-seven fresh samples of tonsillar tissues and 195 samples of adenoid tissues were collected and then examined for the presence of HPV DNA with the polymerase chain reaction (PCR) technique and typing. Laryngeal papilloma specimens from 17 patients obtained during routine debulking procedures were also analyzed and served as positive controls.
All 17 papilloma specimens were positive for HPV DNA and the type was 6 or 11. This result confirmed that the methods used were valid for detecting HPV infection. HPV DNA was detected in 2 of the 177 tonsillar specimens and zero of the 195 adenoid specimens. The two positive samples were confirmed with typing. One was positive for HPV6 and the other for HPV11. Review of the medical records of these two cases confirmed that there were no history of HPV-related diseases. Histologic analysis of their specimens showed lymphoid hyperplasia, no specific changes suggesting HPV infection and no signs of malignancy. The HPV infection rate in upper respiratory tract was 0.8% (2/241).
There is HPV infection in upper respiratory tract in Chinese children without JORRP, but maybe is not sufficient for the formation of JORRP.
本前瞻性研究旨在调查无幼年型复发性呼吸道乳头状瘤病（JORRP）的儿科患者扁桃体切除术和腺样体切除术标本中人类乳头瘤病毒（HPV）的存在情况，以了解HPV感染对儿童上呼吸道的影响。
241例因扁桃体肥大或慢性扁桃体炎接受扁桃体切除术和/或腺样体切除术、无已知JORRP或其他HPV相关疾病的儿科患者纳入本前瞻性研究。收集177份扁桃体组织新鲜样本和195份腺样体组织样本，然后采用聚合酶链反应（PCR）技术检测HPV DNA的存在并进行分型。对17例患者在常规减瘤手术中获得的喉乳头状瘤标本也进行了分析，并作为阳性对照。
所有17份乳头状瘤标本的HPV DNA均为阳性，类型为6型或11型。这一结果证实所采用的方法可有效检测HPV感染。177份扁桃体标本中有2份检测到HPV DNA，195份腺样体标本中未检测到。两份阳性样本经分型确认。一份为HPV6阳性，另一份为HPV11阳性。查阅这两例患者的病历证实无HPV相关疾病史。对其标本进行组织学分析显示为淋巴组织增生，无提示HPV感染的特异性变化，也无恶性迹象。上呼吸道HPV感染率为0.8%（2/241）。
中国无JORRP的儿童上呼吸道存在HPV感染，但可能不足以形成JORRP。
