Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel.
Department of Pathology, Galilee Medical Center, Nahariya, Israel.
Eur Arch Otorhinolaryngol. 2019 Oct;276(10):2865-2871. doi: 10.1007/s00405-019-05543-0. Epub 2019 Jul 12.
The presence of high-risk HPV in non-malignant tonsil tissues from patients who underwent tonsillectomy in the Galilee area might explain the low incidence of HPV-related oropharyngeal malignancy in the country. The aim of this retrospective study was to study the prevalence of human papillomavirus (HPV) in non-malignant tonsillectomy specimens of adults in the Galilee area.
We conducted a retrospective analysis of all tonsil samples in our medical center. Tonsils from patients over 20 years of age who underwent tonsillectomy for the indication of recurrent tonsillitis and sleep apneas were eligible. Samples of formalin-fixed paraffin-embedded (FFPE) tonsillar tissue were tested for the presence of HPV DNA using polymerase chain reaction and by p16 immunohistochemistry.
Of the 71 tonsil samples, age range 20-65, none were positive for HPV DNA. Fifty-two FFPE specimens of tonsil with HPV-positive cancer tested positive by the same method served as positive controls.
HPV DNA is rare in non-malignant tonsil tissues of young adults and adults who underwent tonsillectomy in the Galilee area. Further research should be done in larger cohorts.
在加利利地区接受扁桃体切除术的非恶性扁桃体组织中存在高危型 HPV，这可能解释了该国 HPV 相关性口咽恶性肿瘤发病率低的原因。本回顾性研究的目的是研究加利利地区成人非恶性扁桃体切除术标本中人类乳头瘤病毒（HPV）的流行情况。
我们对我们医疗中心的所有扁桃体样本进行了回顾性分析。年龄在 20 岁以上因复发性扁桃体炎和睡眠呼吸暂停而接受扁桃体切除术的患者有资格入组。使用聚合酶链反应和 p16 免疫组化检测福尔马林固定石蜡包埋（FFPE）扁桃体组织样本中 HPV DNA 的存在。
在 71 例扁桃体样本中，年龄范围为 20-65 岁，均未检测到 HPV DNA。52 例 HPV 阳性癌症的 FFPE 扁桃体标本用相同方法检测为阳性，作为阳性对照。
在加利利地区接受扁桃体切除术的年轻和成年非恶性扁桃体组织中，HPV DNA 很少见。应在更大的队列中进行进一步的研究。
