Divisions of Otolaryngology–Head and Neck Surgery, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
JAMA Otolaryngol Head Neck Surg. 2013 Jan;139(1):82-6. doi: 10.1001/jamaoto.2013.1090.
To assess the validity of the guinea pig as a model for congenital cytomegalovirus (CMV) infection by comparing the effectiveness of detecting the virus by real-time polymerase chain reaction (PCR) in blood, urine, and saliva.
Case-control study.
Academic research.
Eleven pregnant Hartley guinea pigs.
Blood, urine, and saliva samples were collected from guinea pig pups delivered from pregnant dams inoculated with guinea pig CMV. These samples were then evaluated for the presence of guinea pig CMV by real-time PCR assuming 100% transmission.
Thirty-one pups delivered from 9 inoculated pregnant dams and 8 uninfected control pups underwent testing for guinea pig CMV and for auditory brainstem response hearing loss. Repeated-measures analysis of variance demonstrated no statistically significantly lower weight for the infected pups compared with the noninfected control pups. Six infected pups demonstrated auditory brainstem response hearing loss. The sensitivity and specificity of the real-time PCR assay on saliva samples were 74.2% and 100.0%, respectively. The sensitivity of the real-time PCR on blood and urine samples was significantly lower than that on saliva samples.
Real-time PCR assays of blood, urine, and saliva revealed that saliva samples show high sensitivity and specificity for detecting congenital CMV infection in guinea pigs. This finding is consistent with recent screening studies in human newborns. The guinea pig may be a good animal model in which to compare different diagnostic assays for congenital CMV infection.
通过比较实时聚合酶链反应（PCR）在血液、尿液和唾液中检测病毒的有效性，评估豚鼠作为先天性巨细胞病毒（CMV）感染模型的有效性。
病例对照研究。
学术研究。
11 只怀孕的 Hartley 豚鼠。
从接种豚鼠 CMV 的怀孕母鼠所产的豚鼠幼仔中采集血液、尿液和唾液样本。假设 100%的传播率，通过实时 PCR 评估这些样本中是否存在豚鼠 CMV。
从 9 只接种的怀孕母鼠所产的 31 只幼仔和 8 只未感染的对照幼仔中，有 31 只进行了豚鼠 CMV 检测和听觉脑干反应听力损失检测。重复测量方差分析表明，感染组幼仔的体重与未感染对照组幼仔相比无统计学显著差异。6 只感染的幼仔出现听觉脑干反应听力损失。唾液样本实时 PCR 检测的敏感性和特异性分别为 74.2%和 100.0%。实时 PCR 检测血液和尿液样本的敏感性明显低于唾液样本。
血液、尿液和唾液的实时 PCR 分析表明，唾液样本对检测豚鼠先天性 CMV 感染具有较高的敏感性和特异性。这一发现与最近人类新生儿的筛查研究一致。豚鼠可能是一种很好的动物模型，可以比较不同的先天性 CMV 感染诊断检测方法。
