Department of Human Pathology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan,
Brain Tumor Pathol. 2014 Jan;31(1):17-22. doi: 10.1007/s10014-012-0133-x. Epub 2013 Jan 18.
Rosette-forming glioneuronal tumor of the fourth ventricle (RGNT) is a new entity in the WHO 2007 Classification of Tumors of the Central Nervous System. RGNT has two components: neurocytic rosettes and low-grade gliomas. Neurocytic rosettes are conventionally described as consisting of uniform neurocytes. However, some studies have reported rosette-forming tumor cells that expressed glial markers such as Olig2. We indicated the expression of glial markers including Olig2, cyclinD1, glial fibrillary acidic protein (GFAP), and platelet-derived growth factor receptor alpha (PDGFRα) in the neurocytic rosettes in our previous study, and we suggested that these tumor cells had a heterogeneous nature. In this study, we used double and triple immunostaining to demonstrate that these tumor cells have both glial and neuronal characteristics. We found that rosette-forming tumor cells coexpressed Olig2/cyclinD1 and synaptophysin. Furthermore, the cores of the rosettes coexpressed GFAP/PDGFRα in the peripheral zone and synaptophysin in the central zone. These findings imply that rosette-forming tumor cells have a similar nature to neuronal-glial progenitor cells, and we believe that the nomination "neurocytic rosette" may be unsuitable given their heterogeneous nature. Our study appears to clarify some of the properties of RGNT tumor cells and may help elucidate the histogenesis of RGNT.
第四脑室胶神经元性肿瘤（RGNT）是 2007 年世界卫生组织中枢神经系统肿瘤分类中的一个新实体。RGNT 有两个组成部分：神经细胞性玫瑰花结和低级别胶质瘤。神经细胞性玫瑰花结通常被描述为由均匀的神经细胞组成。然而，一些研究报告称，玫瑰花结形成的肿瘤细胞表达神经胶质标志物，如 Olig2。我们在之前的研究中表明，神经细胞性玫瑰花结中表达了包括 Olig2、cyclinD1、胶质纤维酸性蛋白（GFAP）和血小板衍生生长因子受体α（PDGFRα）在内的神经胶质标志物，我们认为这些肿瘤细胞具有异质性。在这项研究中，我们使用双重和三重免疫染色来证明这些肿瘤细胞具有神经胶质和神经元的特征。我们发现玫瑰花结形成的肿瘤细胞共表达 Olig2/cyclinD1 和突触素。此外，玫瑰花结的核心在周围区共表达 GFAP/PDGFRα，在中央区共表达突触素。这些发现表明，玫瑰花结形成的肿瘤细胞具有类似于神经元-神经胶质祖细胞的性质，鉴于其异质性，我们认为“神经细胞性玫瑰花结”的命名可能不合适。我们的研究似乎阐明了 RGNT 肿瘤细胞的一些特性，并可能有助于阐明 RGNT 的组织发生。
