Cellectis Stem Cells , Cellartis AB, Göteborg, Sweden.
Stem Cells Dev. 2013 Apr 15;22(8):1159-61. doi: 10.1089/scd.2012.0668. Epub 2013 Jan 18.
Human pluripotent stem cells (hPSCs), defined by their ability to proliferate indefinitely and the capacity to differentiate into all tissue cell types of the adult, represent a platform for the realization of breakthrough technologies for industrial and regenerative medicine applications. We have witnessed tremendous developments over the last decade related to methods for establishment, maintenance, differentiation, and applications of hPSCs and their derivatives. Despite all progress made in the hPSC field, there are still fundamental issues yet to be resolved. For example, our understanding of the pluripotent state remains limited, which in turn may have substantial consequences on how we interpret and communicate scientific data concerning hPSCs. This brief commentary aims to highlight recent important findings that demonstrate additional levels of complexity to the current assessment of pluripotent stem cell cultures. In addition, these data may help to provide some explanations for the challenges in reproducing hPSC differentiation protocols across laboratories.
人多能干细胞（hPSCs）具有无限增殖和分化为成体所有组织细胞类型的能力，代表了实现工业和再生医学应用突破性技术的平台。在过去的十年中，我们见证了与 hPSCs 及其衍生物的建立、维持、分化和应用相关的方法的巨大发展。尽管 hPSC 领域取得了所有进展，但仍存在一些尚未解决的基本问题。例如，我们对多能状态的理解仍然有限，这反过来可能对我们如何解释和交流有关 hPSCs 的科学数据产生重大影响。本简要评论旨在强调最近的重要发现，这些发现显示了对多能干细胞培养当前评估的更多复杂性。此外，这些数据可能有助于解释在不同实验室复制 hPSC 分化方案所面临的挑战。
