UNESP, Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Professor Francisco Degni 55, Araraquara, SP, Brazil.
Chemosphere. 2013 Apr;91(5):586-93. doi: 10.1016/j.chemosphere.2012.12.027. Epub 2013 Jan 18.
New assays with HepG2 cells indicate that Indigo Carmine (IC), a dye that is widely used as additive in many food and pharmaceutical industries exhibited cytotoxic effects. This work describes the development of a bicomponent nanostructured Ti/TiO2/WO3 electrode prepared by "template" method and investigates its efficiency in a photoelectrocatalytic method by using visible light irradiation and applied potential of 1V. After 2h of treatment there are reduction of 97% discoloration, 62% of mineralization and formation of three byproducts assigned as: 2-amine-5-sulfo-benzoic acid, 2,3-dioxo-14-indole-5-sulfonic acid, and 2-amino-α-oxo-5-sulfo-benzeneacetic acid were identified by HPLC-MS/MS. But, cytotoxicity was completely removed after 120 min of treatment.
用 HepG2 细胞进行的新测定表明，广泛用作许多食品和制药工业添加剂的靛蓝胭脂红 (IC) 表现出细胞毒性作用。本工作描述了通过“模板”法制备的双组分纳米结构 Ti/TiO2/WO3 电极的开发，并通过可见光照射和施加 1V 的应用电势在光电催化方法中研究了其效率。经过 2 小时的处理，有 97%的脱色、62%的矿化和三种副产物的形成，通过 HPLC-MS/MS 鉴定为：2-氨基-5-磺酸苯甲酸、2,3-二氧代-14-吲哚-5-磺酸和 2-氨基-α-氧代-5-磺酸苯乙酸。但是，经过 120 分钟的处理后，细胞毒性完全消除。
