Department of Plant, Soil & Entomological Science, University of Idaho, Moscow, ID 83844, USA.
Biochem Biophys Res Commun. 2013 Feb 15;431(3):501-5. doi: 10.1016/j.bbrc.2013.01.028. Epub 2013 Jan 16.
The highly conserved eukaryotic co-chaperone SGT1 (suppressor of the G2 allele of skp1) is an important signaling component of plant defense responses and positively regulates disease resistance conferred by many resistance (R) proteins. In this study, we investigated the contribution of SGT1 in the Prf-mediated defense responses in both Nicotiana benthamiana and tomato (Solanum lycopersicum). SGT1 was demonstrated to interact with Prf in plant cells by co-immunoprecipitation. The requirement of SGT1 in the accumulation of Prf or autoactive Prf(D1416V) was determined by the degradation of these proteins in N. benthamiana, in which SGT1 was repressed by virus-induced gene silencing (VIGS). Pseudomonas pathogen assay on the SGT1-silenced tomato plants implicates SGT1 is required for the Prf-mediated full resistance to Pseudomonas syringae pv. tomato (Pst). These results suggest that, in both N. benthamiana and tomato, SGT1 contributes to the Prf-mediated defense responses by stabilizing Prf protein via its co-chaperone activity.
高度保守的真核共伴侣 SGT1（抑制 skp1 的 G2 等位基因）是植物防御反应的一个重要信号成分，它正向调节许多抗性（R）蛋白赋予的抗病性。在这项研究中，我们研究了 SGT1 在烟草原生质体和番茄（Solanum lycopersicum）中 Prf 介导的防御反应中的作用。通过共免疫沉淀证实 SGT1 与 Prf 在植物细胞中相互作用。通过病毒诱导的基因沉默（VIGS）抑制烟草原生质体中的 SGT1，确定了 SGT1 在 Prf 或自激活 Prf(D1416V)积累中的必要性。在 SGT1 沉默的番茄植株上进行的假单胞菌病原体测定表明，SGT1 是 Prf 介导的对番茄丁香假单胞菌（Pst）完全抗性所必需的。这些结果表明，在烟草原生质体和番茄中，SGT1 通过其共伴侣活性稳定 Prf 蛋白，从而有助于 Prf 介导的防御反应。
