State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Fitoterapia. 2013 Apr;86:13-8. doi: 10.1016/j.fitote.2013.01.001. Epub 2013 Jan 17.
A water-soluble polysaccharide (MHP-1) isolated from cultured Mortierella hepiali was obtained by hot-water extraction, DEAE-cellulose 52 anion-exchange, and Sephadex G-25 gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, partial hydrolysis with acid, methylation analysis, as well as FTIR and NMR spectroscopy. Data revealed that MHP-1 possessed a backbone consisting of the repeating unit→2)-β-d-Manp-(1, 6)-β-d-Glcp-(1→and 3)-β-d-Manp-(1→, branching at O-4 of→2)-β-d-Manp-(1, and terminated with β-d-Manp. The antitumor effects of MHP-1 on H22 tumor-bearing mice were studied. Results showed that MHP-1 significantly inhibited the growth of implanted H22 tumors in mice in vivo, but not on H22, A549, and Hep-G2 cells in vitro. Serum and spleen cells were separated to evaluate the concentrations of TNF-α by ELISA kit. The release of TNF-α was markedly induced by MHP-1 in a dose-dependent manner.
从培养的霍氏粉褶蕈中分离得到的一种水溶性多糖（MHP-1），采用热水提取、DEAE-纤维素 52 阴离子交换和 Sephadex G-25 凝胶渗透色谱法进行分离纯化。通过 PMP 柱前衍生、酸部分水解、甲基化分析以及傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱等方法对其结构特征进行了研究。结果表明，MHP-1 的主链由重复单元→2)-β-d-Manp-(1,6)-β-d-Glcp-(1→和 3)-β-d-Manp-(1→组成，在→2)-β-d-Manp-(1 的 O-4 处分支，并以β-d-Manp 结尾。研究了 MHP-1 对 H22 荷瘤小鼠的抗肿瘤作用。结果表明，MHP-1 能显著抑制体内植入的 H22 肿瘤的生长，但对体外的 H22、A549 和 Hep-G2 细胞无作用。分离血清和脾细胞，用 ELISA 试剂盒检测 TNF-α 的浓度。结果表明，MHP-1 能以剂量依赖的方式显著诱导 TNF-α 的释放。
