Department of Urology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
Carbohydr Polym. 2013 Jun 20;95(2):615-20. doi: 10.1016/j.carbpol.2013.03.024. Epub 2013 Mar 13.
One water-soluble polysaccharide (PEPw), with an average molecular weight of 2.5×10(4)Da, was isolated from the fruiting bodies of Pleurotus eryngii and subjected to composition analysis and evaluated for the antitumor and immunomodulatory activity. PEPw was composed of arabinose, mannose and galactose in a molar ratio of 1.2:2.3:6.2 and had a backbone mainly consisting of 1,6-linked-Galp, 1,2,6-linked-Galp and 1,4-linked-Manp residues, which was occasionally terminated with terminal-Araf attached to O-2 of 1,2,6-linked-Galp residue. The animal experiment results showed that PEPw significantly increased relative thymus and spleen indices, promoted the spleen lymphocytes proliferation induced by ConA or LPS, elevated the activities of NK cell and CTL in spleen, and increased the serum concentration of TNF-α and IL-2 in Renca tumor-bearing mice. As a result, the tumor growth was significantly inhibited by PEPw treatment at the doses of 50, 100 and 200mg/kg in a dose-dependent manner. These data indicated that the anti-tumor activity of PEPw may be related to the activation of the immune response in tumor-bearing mice.
从杏鲍菇子实体中分离得到一种水溶性多糖（PEPw），其平均分子量为 2.5×10(4)Da。对其进行组成分析，并评价其抗肿瘤和免疫调节活性。PEPw 由阿拉伯糖、甘露糖和半乳糖组成，摩尔比为 1.2:2.3:6.2，其主链主要由 1,6-连接的 Galp、1,2,6-连接的 Galp 和 1,4-连接的 Manp 残基组成，偶尔被末端-Araf 终止，连接在 1,2,6-连接的 Galp 残基的 O-2 上。动物实验结果表明，PEPw 能显著提高荷瘤小鼠的相对胸腺和脾脏指数，促进 ConA 或 LPS 诱导的脾淋巴细胞增殖，提高脾 NK 细胞和 CTL 的活性，增加 Renca 荷瘤小鼠血清中 TNF-α和 IL-2 的浓度。结果表明，PEPw 在 50、100 和 200mg/kg 剂量范围内呈剂量依赖性显著抑制肿瘤生长。这些数据表明，PEPw 的抗肿瘤活性可能与激活荷瘤小鼠的免疫反应有关。
