School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China.
Fitoterapia. 2010 Jul;81(5):397-402. doi: 10.1016/j.fitote.2009.11.008. Epub 2009 Dec 4.
A water-soluble polysaccharide (CPS-2), isolated from the cultured Cordyceps sinensis, was obtained by hot-water extraction, anion-exchange and gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, periodate oxidation, methylation analysis, FTIR and NMR spectroscopy. CPS-2 was found to be mostly of alpha-(1-->4)-D-glucose and alpha-(1-->3)-D-mannose, branched with alpha-(1-->4,6)-D-glucose every twelve residues on average. CPS-2 had a molecular weight of 4.39x10(4) Da. The protective effect of CPS-2 on the model of chronic renal failure was established by fulgerizing kidney. The changes in blood urea nitrogen and serum creatinine revealed that CPS-2 could significantly relieve renal failure caused by fulgerizing kidney.
一种水溶性多糖（CPS-2），从培养的冬虫夏草中提取，通过热水提取、阴离子交换和凝胶渗透色谱法获得。通过 PMP 柱前衍生、高碘酸盐氧化、甲基化分析、FTIR 和 NMR 光谱对其结构特征进行了研究。发现 CPS-2 主要由α-（1-->4）-D-葡萄糖和α-（1-->3）-D-甘露糖组成，每隔十二个残基分支有α-（1-->4,6）-D-葡萄糖。CPS-2 的分子量为 4.39x10（4）Da。通过肾脏闪光法建立 CPS-2 对慢性肾衰竭模型的保护作用。血尿素氮和血清肌酐的变化表明，CPS-2 能显著缓解肾脏闪光引起的肾衰竭。
