Khosravi Ali Reza, Sheikhkarami Mojgan, Shokri Hojjatollah, Sabokbar Azar
Mycology Research Centre, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Arh Hig Rada Toksikol. 2012 Dec;63(4):489-96. doi: 10.2478/10004-1254-63-2012-2237.
We evaluated the ability of randomly amplified polymorphic DNA (RAPD) to type citrinin-producing Penicillium citrinum (P. citrinum) strains recovered from the forest's air in northern Iran. A total of 12 P. citrinum strains (P1-P12) were characterised by citrinin production and random amplification of polymorphic DNA (RAPD) technique. All the strains produced citrinin with levels ranging from 1.5 μg mL(-1) to 39.6 μg mL(-1) (average value: 12.68 μg mL(-1)). Of 11 primers tested, eight primers produced polymorphic amplification patterns. These primers generated a total of 105 reproducible RAPD bands, averaging to 13.1 bands per primer. Dendrogram for each primer indicating the distance of the strains to each other was constructed. RAPD results showed that the collected strains constituted four different clusters. The first cluster included two isolates (P1 and P3). The second cluster included seven isolates (P2, P4, P5, P6, P7, P8, and P10). The third and fourth clusters included one isolate (P9) and two isolates (P11 and P12), respectively. We concluded that RAPD analysis might be used in providing genotypic characters for toxigenic P. citrinum strains typing in epidemiological investigations and public health related risk assessment.
我们评估了随机扩增多态性DNA（RAPD）技术对从伊朗北部森林空气中分离出的产桔霉素的桔青霉（Penicillium citrinum）菌株进行分型的能力。通过桔霉素产量测定和随机扩增多态性DNA（RAPD）技术，对总共12株桔青霉（P1 - P12）进行了特征分析。所有菌株均产生桔霉素，产量范围为1.5 μg mL(-1)至39.6 μg mL(-1)（平均值：12.68 μg mL(-1)）。在测试的11种引物中，有8种引物产生了多态性扩增图谱。这些引物共产生了105条可重复的RAPD条带，平均每条引物产生13.1条带。构建了每个引物的树形图，以表明菌株之间的距离。RAPD结果显示，所收集的菌株分为四个不同的簇。第一簇包括两个分离株（P1和P3）。第二簇包括七个分离株（P2、P4、P5、P6、P7、P8和P10）。第三簇和第四簇分别包括一个分离株（P9）和两个分离株（P11和P12）。我们得出结论，RAPD分析可用于在流行病学调查和公共卫生相关风险评估中为产毒桔青霉菌株分型提供基因型特征。
