Li Taotao, Jiang Guoxiang, Qu Hongxia, Wang Yong, Xiong Yehui, Jian Qijie, Wu Yu, Duan Xuewu, Zhu Xiangrong, Hu Wenzhong, Wang Jiasheng, Gong Liang, Jiang Yueming
Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Zhong Shan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China.
Toxins (Basel). 2017 Feb 21;9(2):69. doi: 10.3390/toxins9020069.
Citrinin is a toxic secondary metabolite of and its contamination in many food items has been widely reported. However, research on the citrinin biosynthesis pathway and its regulation mechanism in is rarely reported. In this study, we investigated the effect of different carbon sources on citrinin production by and used transcriptome analysis to study the underlying molecular mechanism. Our results indicated that glucose, used as the sole carbon source, could significantly promote citrinin production by in Czapek's broth medium compared with sucrose. A total of 19,967 unigenes were annotated by BLAST in Nr, Nt, Swiss-Prot and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Transcriptome comparison between cultured with sucrose and glucose revealed 1085 differentially expressed unigenes. Among them, 610 were upregulated while 475 were downregulated under glucose as compared to sucrose. KEGG pathway and Gene ontology (GO) analysis indicated that many metabolic processes (e.g., carbohydrate, secondary metabolism, fatty acid and amino acid metabolism) were affected, and potentially interesting genes that encoded putative components of signal transduction, stress response and transcription factor were identified. These genes obviously had important impacts on their regulation in citrinin biosynthesis, which provides a better understanding of the molecular mechanism of citrinin biosynthesis by .
桔霉素是[具体物种]的一种有毒次生代谢产物，其在许多食品中的污染已被广泛报道。然而，关于[具体物种]中桔霉素生物合成途径及其调控机制的研究却鲜有报道。在本研究中，我们研究了不同碳源对[具体物种]产桔霉素的影响，并利用转录组分析来研究其潜在的分子机制。我们的结果表明，在察氏肉汤培养基中，与蔗糖相比，以葡萄糖作为唯一碳源可显著促进[具体物种]产桔霉素。通过BLAST在Nr、Nt、Swiss-Prot和京都基因与基因组百科全书（KEGG）数据库中对总共19967个单基因进行了注释。对在蔗糖和葡萄糖中培养的[具体物种]进行转录组比较，发现了1085个差异表达的单基因。其中，与蔗糖相比，在葡萄糖条件下610个上调，475个下调。KEGG通路和基因本体（GO）分析表明，许多代谢过程（如碳水化合物、次生代谢、脂肪酸和氨基酸代谢）受到影响，并且鉴定出了可能有趣的编码信号转导、应激反应和转录因子假定成分的基因。这些基因显然对其在桔霉素生物合成中的调控具有重要影响，这有助于更好地理解[具体物种]合成桔霉素的分子机制。
