Adolphe Merkle Institute, University of Fribourg, Route de l'Ancienne Papeterie, P.O. Box 209, 1723 Marly 1, Switzerland.
Nanoscale. 2013 May 7;5(9):3723-32. doi: 10.1039/c2nr33134a. Epub 2013 Jan 18.
It is known that the nanoparticle-cell interaction strongly depends on the physicochemical properties of the investigated particles. In addition, medium density and viscosity influence the colloidal behaviour of nanoparticles. Here, we show how nanoparticle-protein interactions are related to the particular physicochemical characteristics of the particles, such as their colloidal stability, and how this significantly influences the subsequent nanoparticle-cell interaction in vitro. Therefore, different surface charged superparamagnetic iron oxide nanoparticles were synthesized and characterized. Similar adsorbed protein profiles were identified following incubation in supplemented cell culture media, although cellular uptake varied significantly between the different particles. However, positively charged nanoparticles displayed a significantly lower colloidal stability than neutral and negatively charged particles while showing higher non-sedimentation driven cell-internalization in vitro without any significant cytotoxic effects. The results of this study strongly indicate therefore that an understanding of the aggregation state of NPs in biological fluids is crucial in regards to their biological interaction(s).
已知纳米颗粒与细胞的相互作用强烈依赖于所研究颗粒的物理化学性质。此外，介质密度和粘度会影响纳米颗粒的胶体行为。在这里，我们展示了纳米颗粒与蛋白质的相互作用如何与颗粒的特殊物理化学特性相关，例如胶体稳定性，以及这如何显著影响随后的体外纳米颗粒与细胞的相互作用。因此，我们合成并表征了不同表面带电的超顺磁性氧化铁纳米颗粒。尽管不同颗粒的细胞摄取量有显著差异，但在补充细胞培养基中孵育后，确定了相似的吸附蛋白谱。然而，与中性和负电荷颗粒相比，正电荷纳米颗粒的胶体稳定性显著降低，但在没有明显细胞毒性作用的情况下，体外表现出更高的非沉降驱动的细胞内化。因此，这项研究的结果强烈表明，在考虑纳米颗粒的生物学相互作用时，了解纳米颗粒在生物流体中的聚集状态至关重要。
