Faculty of Civil Engineering, Institute of Environmental and Water Resource Management, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia,
Bioprocess Biosyst Eng. 2013 Oct;36(10):1455-61. doi: 10.1007/s00449-013-0884-8. Epub 2013 Jan 19.
The use of biomaterials or microorganisms in PAHs degradation had presented an eye-catching performance. Pleurotus eryngii is a white rot fungus, which is easily isolated from the decayed woods in the tropical rain forest, used to determine the capability to utilize naphthalene, a two-ring polycyclic aromatic hydrocarbon as source of carbon and energy. In the meantime, biotransformation of naphthalene to intermediates and other by-products during degradation was investigated in this study. Pleurotus eryngii had been incubated in liquid medium formulated with naphthalene for 14 days. The presence of metabolites of naphthalene suggests that Pleurotus eryngii begin the ring cleavage by dioxygenation on C1 and C4 position to give 1,4-naphthaquinone. 1,4-Naphthaquinone was further degraded to benzoic acid, where the proposed terepthalic acid is absent in the cultured extract. Further degradation of benzoic acid by Pleurotus eryngii shows the existence of catechol as a result of the combination of decarboxylation and hydroxylation process. Unfortunately, phthalic acid was not detected in this study. Several enzymes, including manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase are enzymes responsible for naphthalene degradation. Reduction of naphthalene and the presence of metabolites in liquid medium showed the ability of Pleurotus eryngii to utilize naphthalene as carbon source instead of a limited glucose amount.
生物材料或微生物在多环芳烃降解中的应用表现出了引人注目的性能。杏鲍菇是一种白腐真菌，容易从热带雨林中腐烂的木材中分离出来，用于确定利用萘（一种二环多环芳烃）作为碳源和能源的能力。同时，本研究还研究了在降解过程中萘生物转化为中间产物和其他副产物的情况。将杏鲍菇在含有萘的液体培养基中培养 14 天。萘代谢物的存在表明，杏鲍菇通过在 C1 和 C4 位置的双加氧作用开始环裂解，生成 1,4-萘醌。1,4-萘醌进一步降解为苯甲酸，其中所提出的对苯二甲酸在培养提取物中不存在。杏鲍菇进一步降解苯甲酸表明存在儿茶酚，这是由于脱羧和羟化过程的结合。不幸的是，在本研究中未检测到邻苯二甲酸。几种酶，包括锰过氧化物酶、木质素过氧化物酶、漆酶、1,2-加氧酶和 2,3-加氧酶，是负责萘降解的酶。萘的还原和代谢物在液体培养基中的存在表明杏鲍菇有能力将萘作为碳源利用，而不是仅利用有限的葡萄糖。
