Hadibarata Tony, Kristanti Risky Ayu, Hamdzah Myzairah
Institute of Environmental and Water Resources Management, Faculty of Civil Engineering, Universiti Teknologi Malaysia, UTM Skudai, Johor Bahru, Malaysia.
Biotechnol Appl Biochem. 2014 Mar-Apr;61(2):126-33. doi: 10.1002/bab.1155. Epub 2014 Feb 22.
Major concern about the presence of fluoranthene, which consists of four fused benzene rings, in the environment has been raised in the past few years due to its toxic, mutagenic, and persistent organic pollutant properties. In this study, we investigated the removal of fluoranthene under static and agitated conditions. About 89% fluoranthene was removed within 30 days under the agitated condition, whereas under the static condition, only 54% fluoranthene was removed. We further investigated the behavior and mechanism of fluoranthene biosorption and biotransformation by Pleurotus eryngii F032 to accelerate the elimination of fluoranthene. The optimum conditions for the elimination of fluoranthene by P. eryngii F032 included a temperature of 35 °C, pH 3, 0.2% inoculum concentration, and a C/N ratio of 16. Under these conditions at the initial fluoranthene concentration of 10 mg/L, more than 95% of fluoranthene was successfully removed within 30 days. Of those factors influencing the biodegradation of fluoranthene, salinity, glucose, and rhamnolipid content were of the greatest importance. Degradation metabolites identified using gas chromatography-mass spectrometry were 1-naphthalenecarboxylic acid and salicylic acid, suggesting possible metabolic pathways. Finally, it can be presumed that the major mechanism of fluoranthene elimination by white-rot fungi is to mineralize polycyclic aromatic hydrocarbons via biotransformation enzymes like laccase.
在过去几年中，由于荧蒽具有毒性、致突变性和持久性有机污染物的特性，其在环境中的存在引发了人们的重大关注。荧蒽由四个稠合苯环组成。在本研究中，我们调查了在静态和搅拌条件下荧蒽的去除情况。在搅拌条件下，30天内约89%的荧蒽被去除，而在静态条件下，仅54%的荧蒽被去除。我们进一步研究了杏鲍菇F032对荧蒽的生物吸附和生物转化行为及机制，以加速荧蒽的消除。杏鲍菇F032消除荧蒽的最佳条件包括温度35℃、pH值3、接种物浓度0.2%以及碳氮比16。在这些条件下，当初始荧蒽浓度为10mg/L时，30天内超过95%的荧蒽被成功去除。在影响荧蒽生物降解的那些因素中，盐度、葡萄糖和鼠李糖脂含量最为重要。使用气相色谱 - 质谱法鉴定的降解代谢产物为1 - 萘甲酸和水杨酸，这表明了可能的代谢途径。最后，可以推测白腐真菌消除荧蒽的主要机制是通过漆酶等生物转化酶将多环芳烃矿化。
