Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia.
Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 567-0871, Japan.
Fungal Biol. 2014 Feb;118(2):222-7. doi: 10.1016/j.funbio.2013.11.013. Epub 2013 Dec 19.
The white-rot fungus Pleurotus eryngii F032 showed the capability to degrade a three fused-ring aromatic hydrocarbons fluorene. The elimination of fluorene through sorption was also investigated. Enzyme production is accompanied by an increase in biomass of P. eryngii F032 during degradation process. The fungus totally degraded fluorine within 23 d at 10-mg l(-1) solution. Fluorene degradation was affected with initial fluorene concentrations. The highest enzyme activity was shown by laccase in the 10-mg l(-1) culture after 30 d of incubation (1620 U l(-1)). Few activities of enzymes were observed in the fungal cell at the varying concentration of fluorene. Three metabolic were detected and separated in ethylacetate extract, after isolated by column chromatography. The metabolites, 9-fluorenone, phthalic acid, and benzoic acid were identified using UV-vis spectrophotometer and gas chromatography-mass spectrometry (GC-MS). The results show the presence of a complex mechanism for the regulation of fluorene-degrading enzymes.
白腐菌糙皮侧耳 F032 显示出降解三种稠环芳烃芴的能力。还研究了通过吸附去除芴。在降解过程中，酶的产生伴随着糙皮侧耳 F032 生物量的增加。真菌在 10-mg l(-1)溶液中 23 天内完全降解了芴。芴的初始浓度对芴的降解有影响。在 30 天的培养后，在 10-mg l(-1)的培养物中，漆酶表现出最高的酶活性（1620 U l(-1)）。在不同浓度的芴中，在真菌细胞中观察到很少的酶活性。在柱层析分离后，从乙酸乙酯提取物中检测到并分离出三种代谢产物。使用紫外可见分光光度计和气相色谱-质谱联用仪（GC-MS）鉴定了代谢产物 9-芴酮、邻苯二甲酸和苯甲酸。结果表明，存在一种复杂的芴降解酶调控机制。
