Department of Radiation Medicine, Preventive Medicine College, Fourth Military Medical University, Xi'an 710032, Shaanxi, China.
Biomed Environ Sci. 2013 Feb;26(2):128-37. doi: 10.3967/0895-3988.2013.02.007.
To study the effect of electromagnetic pulse (EMP) exposure on permeability of in vitro blood-brain-barrier (BBB) model.
An in vitro BBB model, established by co-culturing brain microvascular endothelial cells (BMVEC) and astroglial cells (AC) isolated from rat brain, was exposed to EMP at 100 kV/m and 400 kV/m, respectively. Permeability of the model was assayed by measuring the transendothelial electrical resistance (TEER) and the horseradish peroxidase (HRP) transmission at different time points. Levels of BBB tight junction-related proteins were measured at 0, 1, 2, 4, 8, 12, 16, 20, 24 h after EMP exposure by Western blotting.
The TEER level was lower in BBB model group than in control group at 12 h after EMP, exposure which returned to its normal level at 24 h. The 24 h recovery process was triphasic and biphasic respectively after EMP exposure at 100 kV/m and 400 kV/m. Following exposure to 400 kV/m EMP, the HRP permeability increased at 1-12 h and returned to its normal level at 24 h. Western blotting showed that the claudin-5 and ZO-1 protein levels were changed after EMP exposure.
EMP exposure at 100 kV/m and 400 kV/m can increase the permeability of in vitro BBB model and BBB tight junction-related proteins such as ZO-1 and claudin-5 may change EMP-induced BBB permeability.
研究电磁脉冲（EMP）暴露对体外血脑屏障（BBB）模型通透性的影响。
采用共培养大鼠脑微血管内皮细胞（BMVEC）和星形胶质细胞（AC）的方法建立体外 BBB 模型，分别用 100 kV/m 和 400 kV/m 的 EMP 进行暴露。在不同时间点通过测量跨内皮电阻（TEER）和辣根过氧化物酶（HRP）的传递来检测模型的通透性。采用 Western blot 法在 EMP 暴露后 0、1、2、4、8、12、16、20、24 h 测量 BBB 紧密连接相关蛋白的水平。
EMP 暴露 12 h 后 BBB 模型组的 TEER 水平低于对照组，24 h 时恢复正常。EMP 暴露 100 kV/m 和 400 kV/m 后，24 h 的恢复过程分别呈三相和双相。暴露于 400 kV/m EMP 后，HRP 通透性在 1-12 h 内增加，24 h 时恢复正常。Western blot 显示 EMP 暴露后紧密连接相关蛋白 claudin-5 和 ZO-1 的蛋白水平发生变化。
EMP 暴露 100 kV/m 和 400 kV/m 可增加体外 BBB 模型的通透性，EMP 诱导的 BBB 通透性改变可能与 ZO-1 和 claudin-5 等 BBB 紧密连接相关蛋白有关。
