Department of Radiation Protection Medicine, School of Preventive Medicine, Air Force Medical University, Xi'an, China.
Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an, China.
Front Public Health. 2023 Mar 16;11:1087161. doi: 10.3389/fpubh.2023.1087161. eCollection 2023.
Recently, concerns about the combined effects of electromagnetic field (EMF) in daily living and occupational environment are rapidly growing.
In this study, we investigated the combined effects of 1-week exposure to electromagnetic pulse (EMP) at 650 kV/m for 1,000 pulses and 4.9 GHz radiofrequency (RF) at 50 W/m for 1 h/d in male mice. Open field test, tail suspension test and Y-maze were applied to evaluate anxiety, depression-like behaviors and spatial memory ability, respectively.
It was found that compared with Sham group, combined exposure to EMP and RF induced anxiety-like behavior, increased the level of serum S100B and decreased the level of serum 5-HT. The results of quantitative proteomic and KEGG analysis showed that the differentially expressed proteins in hippocampus were enriched in Glutamatergic and GABAergic synapse after combined exposure group, which were verified by western blot. In addition, an obvious histological alteration and autophagy-associated cell death were observed in amygdala instead of hippocampus after combined exposure to EMP and 4.9 GHz RF.
Combined exposure to EMP and 4.9 GHz RF could induce emotional behavior alteration, which might be associated with Glutamatergic and GABAergic synapse system of hippocampus and autophagy in amygdala.
最近，人们对日常生活和职业环境中电磁场（EMF）的综合影响的担忧迅速增加。
在这项研究中，我们研究了男性小鼠暴露于 1 周 650 kV/m 的电磁脉冲（EMP）1000 次和 4.9 GHz 射频（RF）50 W/m 1 小时/天的综合影响。应用旷场试验、悬尾试验和 Y 迷宫分别评估焦虑、抑郁样行为和空间记忆能力。
与假处理组相比，EMP 和 RF 联合暴露诱导焦虑样行为，增加血清 S100B 水平，降低血清 5-HT 水平。定量蛋白质组学和 KEGG 分析结果显示，联合暴露组海马体中差异表达的蛋白质在谷氨酸能和 GABA 能突触中富集，通过 Western blot 得到验证。此外，在 EMP 和 4.9 GHz RF 联合暴露后，杏仁核中观察到明显的组织学改变和自噬相关细胞死亡，而海马体中未观察到。
EMP 和 4.9 GHz RF 的联合暴露会导致情绪行为改变，这可能与海马体的谷氨酸能和 GABA 能突触系统以及杏仁核中的自噬有关。
