Departamento de Biologia Molecular e Biotecnologia, Centro de Biotecnologia da Universidade Federal do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Stem Cells Dev. 2013 May 1;22(9):1455-76. doi: 10.1089/scd.2012.0637. Epub 2013 Jan 22.
Stem cells (SC) are able to self-renew and to differentiate into many types of committed cells, making SCs interesting for cellular therapy. However, the pool of SCs in vivo and in vitro consists of a mix of cells at several stages of differentiation, making it difficult to obtain a homogeneous population of SCs for research. Therefore, it is important to isolate and characterize unambiguous molecular markers that can be applied to SCs. Here, we review classical and new candidate molecular markers that have been established to show a molecular profile for human embryonic stem cells (hESCs), mesenchymal stem cells (MSCs), and hematopoietic stem cells (HSCs). The commonly cited markers for embryonic ESCs are Nanog, Oct-4, Sox-2, Rex-1, Dnmt3b, Lin-28, Tdgf1, FoxD3, Tert, Utf-1, Gal, Cx43, Gdf3, Gtcm1, Terf1, Terf2, Lefty A, and Lefty B. MSCs are primarily identified by the expression of CD13, CD29, CD44, CD49e, CD54, CD71, CD73, CD90, CD105, CD106, CD166, and HLA-ABC and lack CD14, CD31, CD34, CD45, CD62E, CD62L, CD62P, and HLA-DR expression. HSCs are mainly isolated based on the expression of CD34, but the combination of this marker with CD133 and CD90, together with a lack of CD38 and other lineage markers, provides the most homogeneous pool of SCs. Here, we present new and alternative markers for SCs, along with microRNA profiles, for these cells.
干细胞 (SC) 具有自我更新和分化为多种定向细胞的能力，这使得 SC 成为细胞治疗的研究热点。然而，体内和体外的 SC 群体由处于不同分化阶段的细胞混合组成，这使得很难获得同质的 SC 群体用于研究。因此，分离和鉴定可应用于 SC 的明确分子标记物非常重要。在这里，我们回顾了已经建立的经典和新的候选分子标记物，这些标记物可用于显示人类胚胎干细胞 (hESC)、间充质干细胞 (MSC) 和造血干细胞 (HSC) 的分子特征。胚胎 ESCs 常用的标记物有 Nanog、Oct-4、Sox-2、Rex-1、Dnmt3b、Lin-28、Tdgf1、FoxD3、Tert、Utf-1、Gal、Cx43、Gdf3、Gtcm1、Terf1、Terf2、Lefty A 和 Lefty B。MSC 主要通过表达 CD13、CD29、CD44、CD49e、CD54、CD71、CD73、CD90、CD105、CD106、CD166 和 HLA-ABC 来鉴定，并且缺乏 CD14、CD31、CD34、CD45、CD62E、CD62L、CD62P 和 HLA-DR 的表达。HSC 主要基于 CD34 的表达进行分离，但将该标记物与 CD133 和 CD90 结合，同时缺乏 CD38 和其他谱系标记物，可提供最同质的 SC 群体。在这里，我们为这些细胞提供了新的和替代的 SC 标记物以及 microRNA 图谱。
