Center of Neuroscience Excellence, Louisiana State University Health Science Center, New Orleans, Louisiana.
J Histochem Cytochem. 2014 Apr;62(4):276-85. doi: 10.1369/0022155414520710. Epub 2014 Jan 7.
Various types of endogenous stem cells (SCs) participate in wound healing in the skin at different anatomical locations. SCs need to be identified through multiple markers, and this is usually performed using flow cytometry. However, immunohistological identification of endogenous stem cells in the skin at different anatomical locations by co-staining multiple SC markers has been seldom explored. We examined the immunohistological localization of four major types of SCs in wounded skin by co-staining for their multiple markers. Hematopoietic SCs were co-stained for Sca1 and CD45; mesenchymal SCs for Sca1, CD29, and CD106; adipose SCs for CD34, CD90, and CD105; and endothelial progenitor cells and their differentiated counterparts were co-stained for CD34, Tie2, and von Willebrand factor. We found Sca1(+)CD45(+) SCs in the epidermis, dermis and hypodermis of wounded skin. Sca1(+)CD29(+) and Sca1(+)CD106(+) mesenchymal SCs, CD34(+)CD105(+), CD34(+)CD90(+), and CD90(+)CD105(+) adipose SCs, as well as CD34(+)Tie2(+) endothelial progenitor cells were also located in the epidermis, dermis, and hypodermis. This study demonstrates the feasibility of using immunohistological staining to determine the location of SCs in wounded skin and the intracellular distribution of their molecular markers.
各种类型的内源性干细胞（SCs）在不同解剖部位的皮肤参与伤口愈合。需要通过多个标记物来识别SCs，这通常通过流式细胞术来完成。然而，通过共同染色多个 SC 标记物来免疫组织化学鉴定不同解剖部位皮肤中的内源性干细胞的研究还很少。我们通过共同染色它们的多个标记物来检查受伤皮肤中四种主要类型的 SC 的免疫组织化学定位。造血干细胞共同染色 Sca1 和 CD45；间充质干细胞共同染色 Sca1、CD29 和 CD106；脂肪干细胞共同染色 CD34、CD90 和 CD105；内皮祖细胞及其分化的对应物共同染色 CD34、Tie2 和血管性血友病因子。我们发现 Sca1(+)CD45(+)SCs 存在于受伤皮肤的表皮、真皮和皮下组织中。Sca1(+)CD29(+)和 Sca1(+)CD106(+)间充质干细胞、CD34(+)CD105(+)、CD34(+)CD90(+)和 CD90(+)CD105(+)脂肪干细胞以及 CD34(+)Tie2(+)内皮祖细胞也存在于表皮、真皮和皮下组织中。这项研究表明，使用免疫组织化学染色来确定受伤皮肤中 SC 的位置及其分子标记物的细胞内分布是可行的。
