Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immunodrugs of Education Ministry of China, Hefei, China.
Scand J Clin Lab Invest. 2013 Mar;73(2):141-7. doi: 10.3109/00365513.2012.756120. Epub 2013 Jan 22.
The study was promoted to probe into the effect of BLyS on the activity of peripheral B lymphocytes mediated by BLyS receptors in patients with systemic lupus erythematosus (SLE). Thirty new-onset patients having fulfilled the American College of Rheumatology (ACR) revised criteria for the diagnosis of SLE. Twenty age-matched healthy volunteers were recruited for this study. Peripheral blood samples were used to analyze the expression of BLyS protein and related receptors (BR3, TACI), to detect peripheral B cell subpopulations of different phases. Clinical disease activity was evaluated according to the systemic lupus erythematosus disease activity index (SLEDAI-2000). The percentage of CD19(+)CD5(+), CD19(+)CD27(+), CD19(+)CD38(+) and total CD19(+) in the peripheral blood is significantly higher in SLE patients than that in healthy controls (p < 0.01). BLyS concentrations and TACI expression are up-regulated in SLE patients (p < 0.01) while BR3 showed no differences between the two groups (p > 0.05). BLyS concentrations and TACI MFI both showed positive correlation with SLEDAI (r(2) = 0.391, p < 0.001, r(2) = 0.339, p = 0.001), whereas BR3 expression showed no relationship with SLEDAI. The disorders of peripheral B cell subsets maybe reflect the effect of BLyS on the activity of peripheral B lymphocytes mediated by BLyS receptors. The significant up-regulation of BLyS and its receptors, especially TACI may serve as a target for the treatment of SLE.
该研究旨在探讨 B 淋巴细胞刺激因子（BLyS）对系统性红斑狼疮（SLE）患者外周 B 淋巴细胞活性的影响。该研究纳入了 30 例符合美国风湿病学会（ACR）修订的 SLE 诊断标准的新发病例，以及 20 例年龄匹配的健康志愿者。采用外周血样本分析 BLyS 蛋白及其相关受体（BR3、TACI）的表达，检测不同阶段的外周 B 细胞亚群。根据系统性红斑狼疮疾病活动指数（SLEDAI-2000）评估临床疾病活动度。SLE 患者外周血中 CD19(+)CD5(+)、CD19(+)CD27(+)、CD19(+)CD38(+)和总 CD19(+)细胞的比例明显高于健康对照组（p < 0.01）。SLE 患者 BLyS 浓度和 TACI 表达上调（p < 0.01），而 BR3 两组间无差异（p > 0.05）。BLyS 浓度和 TACI MFI 均与 SLEDAI 呈正相关（r²=0.391，p < 0.001，r²=0.339，p=0.001），而 BR3 表达与 SLEDAI 无相关性。外周 B 细胞亚群的紊乱可能反映了 BLyS 对 BLyS 受体介导的外周 B 淋巴细胞活性的影响。BLyS 及其受体的显著上调，尤其是 TACI，可能成为治疗 SLE 的靶点。
