1型糖尿病（DM1）年轻患者抗氧化应激和血管病变的保护机制。
Protective mechanisms against oxidative stress and angiopathy in young patients with diabetes type 1 (DM1).
作者信息
Koutroumani Nikolitsa, Partsalaki Ioanna, Lamari Fotini, Dettoraki Athina, Gil Andrea Paola Rojas, Karvela Alexia, Kostopoulou Eirini, Spiliotis Bessie E
机构信息
Department of Pediatrics, University of Patras School of Medicine, Patras, Achaia, Greece.
出版信息
J Pediatr Endocrinol Metab. 2013;26(3-4):309-17. doi: 10.1515/jpem-2012-0183.
OBJECTIVE
Advanced glycation end-products (AGEs) via their receptor, RAGE, are involved in diabetic angiopathy. Soluble RAGE, an inhibitor of this axis, is formed by enzymatic catalysis (sRAGE) or alternative splicing (esRAGE). Malondialdehyde (MDA) is an oxidative stress marker, and ferric reducing ability of plasma (FRAP) is an anti-oxidant capacity marker.
METHODS
In isolated mononuclear blood cells from 110 DM1-patients (P) and 124 controls (C) (4-29 years) RAGE mRNA (g) and protein expression (pe) were measured by RT-PCR and Western immunoblotting, respectively. Plasma levels of CML (AGEs) and sRAGE were measured by ELISA, MDA by flurometry and FRAP according to 'Benzie and Strain'.
RESULTS
P showed: (i) higher g of RAGE, especially in p>13 years of age and >5 years DM1, (ii) increased pe of esRAGE in DM1>5 years and (iii) increased FRAP and MDA.
CONCLUSIONS
The increased esRAGE and FRAP with increased levels of CML and MDA possibly reflects a protective response against the formation of diabetic complications in these young diabetic patients.
目的
晚期糖基化终产物（AGEs）通过其受体RAGE参与糖尿病血管病变。可溶性RAGE是该轴的一种抑制剂，可通过酶催化（sRAGE）或可变剪接（esRAGE）形成。丙二醛（MDA）是氧化应激标志物，血浆铁还原能力（FRAP）是抗氧化能力标志物。
方法
分别通过逆转录聚合酶链反应（RT-PCR）和Western免疫印迹法测定110例1型糖尿病患者（P）和124例对照者（C）（4 - 29岁）分离的单核血细胞中RAGE信使核糖核酸（g）和蛋白表达（pe）。采用酶联免疫吸附测定法（ELISA）测定血浆羧甲基赖氨酸（CML，一种AGEs）和sRAGE水平，采用荧光测定法测定MDA水平，并根据“本齐和斯特林”方法测定FRAP。
结果
患者表现为：（i）RAGE的g值较高，尤其是在年龄大于13岁且患1型糖尿病超过5年的患者中；（ii）患1型糖尿病超过5年的患者中esRAGE的pe增加；（iii）FRAP和MDA增加。
结论
esRAGE和FRAP增加，同时CML和MDA水平升高，这可能反映了这些年轻糖尿病患者针对糖尿病并发症形成的一种保护反应。
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