晚期糖基化终末产物可溶性受体与男性不育
Soluble receptor for advanced glycation end products in male infertility.
作者信息
Charalampidou S, Simitsopoulou Μ, Skoura L, Tziomalos K, Koulourida V, Goulis D G
机构信息
Second Department of Obstetrics and Gynecology, Hippokration Hospital of Thessaloniki, Thessaloniki, Greece.
Laboratory of Infectious Diseases, 3 Department of Pediatrics, Hippokration Hospital of Thessaloniki, Thessaloniki, Greece.
出版信息
Hippokratia. 2017 Jan-Mar;21(1):19-24.
BACKGROUND
Soluble receptor of advanced glycation end-products (sRAGE) is widely expressed in various organs including male genital tract and spermatozoa. Their regulation depends on many simultaneous conditions that may occur. Male fertility is a multifactorial condition which is influenced by various parameters, some of which are detrimental to the spermatozoa. The aim of this study was to detect possible differences in sRAGE concentrations between serum and seminal plasma of infertile men, compared to fertile men population.
METHODS
Seventy-five men were included in the study and divided into three groups: a group of fertile controls (age 34.8 ± 4.6 years, n =12) and two groups of men from infertile couples (age 36.1 ± 5.2 years) with normal (NS, n =10) and abnormal (AS, n =53) semen parameters, respectively. sRAGE was measured by ELISA in serum, and seminal plasma samples of all participants and biochemical, hormonal examinations, as well as anthropometric characteristics, were co-evaluated. Data were statistically analyzed using the chi-square test and the independent samples t-test. A two-tailed p <0.05 was considered significant.
RESULTS
Serum sRAGE levels of fertile men were higher than those of men of infertile couples with AS or NS semen parameters (2,061 ± 884 pg/ml vs 1,673 ± 613 pg/ml and 1,411 ± 405 pg/ml, respectively; p <0.058). Seminal plasma sRAGE levels in fertile men were similar to the ones measured in both groups of men from infertile couples AS and NS (327 ± 81 pg/ml vs 322 ± 162 - 413 ± 207 pg/ml; p =0.197). Interestingly, the seminal plasma sRAGE levels in those three groups were significantly lower than the corresponding serum sRAGE levels (p <0.001).
CONCLUSION
Serum and seminal plasma sRAGE concentrations seem to show variations worth considering between fertile and infertile men. Moreover, further research is required to elucidate the role of the sRAGEs and oxidative stress in male infertility. HIPPOKRATIA 2017, 21(1): 19-24.
背景
晚期糖基化终产物可溶性受体（sRAGE）在包括男性生殖道和精子在内的多种器官中广泛表达。其调节取决于许多可能同时出现的条件。男性生育能力是一种多因素状况，受多种参数影响，其中一些对精子有害。本研究的目的是检测与生育男性群体相比，不育男性血清和精浆中sRAGE浓度的可能差异。
方法
75名男性纳入研究并分为三组：一组生育对照组（年龄34.8±4.6岁，n = 12）和两组来自不育夫妇的男性（年龄36.1±5.2岁），分别具有正常（NS，n = 10）和异常（AS，n = 53）精液参数。通过ELISA法测定所有参与者血清和精浆中的sRAGE，并对生化、激素检查以及人体测量特征进行综合评估。数据采用卡方检验和独立样本t检验进行统计学分析。双侧p<0.05被认为具有统计学意义。
结果
生育男性的血清sRAGE水平高于精液参数异常或正常的不育夫妇男性（分别为2,061±884 pg/ml vs 1,673±613 pg/ml和1,411±405 pg/ml；p<0.058）。生育男性精浆中的sRAGE水平与精液参数异常和正常的两组不育夫妇男性中测得的水平相似（327±81 pg/ml vs 322±162 - 413±207 pg/ml；p = 0.197）。有趣的是，这三组精浆中的sRAGE水平均显著低于相应的血清sRAGE水平（p<0.001）。
结论
血清和精浆中sRAGE浓度在生育和不育男性之间似乎存在值得考虑的差异。此外，需要进一步研究以阐明sRAGE和氧化应激在男性不育中的作用。《希波克拉底》2017年，21(1): 19 - 24。
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